摘要
目的:传统的全细胞膜片钳技术在离子通道电流的记录中存在机械稳定性差,对细胞的损伤大,以及胞内液的被渗析影响与细胞内信号转导和离子通道调控有关的第二信使物质的正常运行。而穿孔全细胞膜片钳技术应用二性霉素B或制霉菌素在细胞膜上形成特定的孔道,选择性地允许一些离子和大分子物质,从而使细胞内环境保持相对稳定,在一定程度上弥补了上述缺陷,实验成功率也相应提高。本文就穿孔膜片钳技术在全细胞离子通道电流记录中的应用进行探讨。
Conventional whole-cell patch recording suffers from more damaging to cells, less mechanical stability ,as well as. the drawbacks related to the dialysis of the cell interior with the pipette solution ,which washes out cytoplasmic biochemicals required for channel activities and second messenger-mediated responses,and disrupts intracellular Ca^2＋ buffering systems. While perforated patch recording technique used antibiotic amphotericin B or nystatin to form small pores in the membrane under the patch pipette. These pores allow small monovalent ions to pass hut prevent the movement of larger molecules. Thus , one has electrical acess to the entire cell with minimal dialysis of the cytoplasm , avoiding the loss of normal intracellular constituents that occurs in the conventional whole-cell mode of patch-clamp recording. The aim of this paper is to outline the properties of perforated patch recording and its applicafions.
出处
《四川生理科学杂志》
2006年第2期62-65,共4页
Sichuan Journal of Physiological Sciences
基金
国家自然科学基金资助课题No.30370527
