摘要
用膜片钳单通道技术 ,观察在细胞膜内侧加入甲基莲心碱 (Nef)后 K+通道特性的改变 ,以研究Nef对豚鼠心室肌细胞及猪冠状动脉平滑肌细胞K+通道的作用 .结果 :Nef可使心肌细胞及冠脉平滑肌细胞上 K+通道开放概率 (PO)降低 . 1 0 ,2 0μmol· L-1Nef分别使心肌细胞 K+通道降低 (50± 32 ) %和 (96± 5) % ,30 ,50 μmol· L-1的 Nef分别使猪冠脉平滑肌细胞上 K+通道 PO 降低 (51±2 1 ) %和 (71± 1 4) % .结果表明 ,Nef可从通道内口阻断心肌及平滑肌细胞上 K+通道 。
To study the effects of neferine (Nef) on K + channels in guinea pig ventricular cells and porcine coronary artery smooth muscle cells (SMC), the changes of the properties of single K + channel after application of Nef were investigated using inside out patch clamp technique. Results indicated that Nef involved a blockade of K + channel both in guinea pig ventricular cells and porcine coronary artery SMC. Application of Nef 10, 20 μmol·L -1 from inner side of membrane of ventricular cells decreased the open probability(P O) of the channel by (50±32)% and (96±5)%, respectively, whereas in coronary artery SMC 30 and 50 μmol·L -1 Nef decreased P O of K + channel by (51±21)% and (71±14)%, respectively. This study suggests that Nef block K + channel from inner mouth of the channel both in ventricular cells and coronary artery SMC, and the blocking potency in the former be stronger than in the later.
出处
《中国药理学与毒理学杂志》
CSCD
北大核心
2000年第6期405-410,共6页
Chinese Journal of Pharmacology and Toxicology
基金
四川省教委资助课题(97-12)
关键词
甲基莲心碱
膜片钳技术
钾通道
心肌
冠状血管
neferine
patch clamp technique
potassium channels
myocardium
coronary vessels
