摘要
目的观察自组装peptide胶对神经干细胞（NSCs）在大鼠急性期损伤脊髓中的细胞分化的影响。方法分离、培养和鉴定大鼠NSCs与自组装peptide胶共培养；SD大鼠25只采用NYU—Ⅱ型脊髓打击器制作T10脊髓损伤模型；急性期于损伤脊髓区分别行注射移植，其中联合细胞移植组（n=10）、单纯细胞移植组（n=10）及对照组（n=5）。术后第2、4、8周取损伤部位脊髓，免疫组织化学染色检测移植细胞的分化。结果成功建立大鼠NSCs的体外培养体系；移植的NSCs在大鼠脊髓内存活超过8周，单纯移植组、细胞分化比例较低，分化的NSCs主要为表达胶质纤维酸性蛋白（GFAP）抗原细胞，未见NSCs分化为表达微管相关蛋白（MAP2）标志抗原细胞；联合移植组，细胞分化比例较高；NSCs可分化为表达MAP2、Oligo、GFAP标志抗原细胞。结论自组装peptide胶能够提高神经干细胞在大鼠急性期损伤脊髓中的分化比率，并有部分细胞可分化为表达神经元特异抗原的细胞。
Objective To study the influence of self-assembled peptide nanofiber scaffold on the proliferation of the neural stem cells （NSCs） transplanted in rats＇ injured spinal cord in the acute phase. Methods NSCs in rats were isolated, cultured and identified, and co-cultured with self-assembled peptide nanofiber scaffold. Twenty-five SD rats were hit with NYU-Ⅱ strike machine at T10 to establish rats＇ spinalcord injury model. Transplant cells and peptide with injection in acute phase of injured spinal cords, in which the united cell transplantation group （ n = 10 ）, a simple cell transplantation group （ n = 10 ） and the control group （ n = 5 ）. 2,4,8 weeks after the transplantation,imInunohistoehemistry was made to test the cells＇ divi- sion. Results The rats＇ NSCs culture system was successfully established in vitro. NSCs transplanted in the injured spinal cords could live more than 8 weeks. In simply transplantation group, NSCs had a lower proportion of cell differentiation, and no mierotuhule-associated protein 2 （MAP2） marked cells differentiation happened, and a little giial fibrillary acidic protein （GFAP） marked cells differentiation. In united transplantation group, NSCs had a higher proportion of ceils differentiation, and could differentiate into MAP2/Oligo/GFAP marked cells. Conclusion Self-assembled peptide nanofiber scaffold could improve the neural stem cells＇ differentiation proportion in the acute phase of rats＇injured spinal cord, and some of the cells could differentiate into neuron-specific antigen cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第4期512-514,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30800634）
广东省自然科学基金资助项目（7001603）
关键词
脊髓损伤
神经干细胞
组织工程
分化
Spinal cord injury
Neural stem cells
Tissue engineering
Differentiation
