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转基因雪旺细胞与壳聚糖的相容性 被引量:2

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摘要 大量动物实验证实:将体外培养的雪旺细胞(SC)移植入脊髓损伤区能促进神经元再生,但SC在体外培养和移植过程中,缺乏有效载体。本实验拟建立SC体外分离培养的方法,将神经生长因子(NGF)基因转入SC,使用酶联免疫吸附试验(ELISA)方法检测NGF在转染前后的分泌情况;使用壳聚糖作为载体,种植转基因SC,并对两者的相容性进行观察,探讨转基因和壳聚糖在脊髓损伤治疗中的作用。
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2006年第9期1146-1146,共1页 Chinese Journal of Experimental Surgery
基金 国家自然科学基金(39900151)
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