摘要
目的探讨Klotho蛋白及自噬相关蛋白微管相关蛋白1轻链3（LC3）在胃癌组织中的表达及意义。方法免疫组织化学超敏二步法分别检测68例胃癌组织及40例配对癌旁组织中Klotho及LC3蛋白的表达，分析两者表达与各临床病理因素的关系以及两者表达的相关性。结果Klotho在胃癌组织中的阳性表达率为41．2％（28／68）低于癌旁组织的67．5％（26／40，P〈0．05），且与肿瘤大小、分化程度及TNM分期相关（P〈0．05）。LC3在胃癌组织中的阳性表达率为45．6％（31／68）低于癌旁组织的70．0％（28／40，P〈0．05），且与肿瘤大小、分化程度及TNM分期相关（P〈0．05）。Klotho和LC3在胃癌组织中的表达呈正相关（P〈0．05）。结论胃癌组织自噬活性降低，Klotho及LC3表达下调可能参与了胃癌的发生发展过程。
Objective To investigate the expression and significance of Klotho protein and autophagy related protein microtubule - associated protein 1 light chain 3 （ LC3 ） in gastric cancer tissues. Meth- ods The expression of LC3 and Klotho protein in 68 cases of gastric cancer tissues and 40 cases of gastric cancer tissues, gastric cancer tissues were detected by immunohistochemical method, and the relationship between the expression of the two proteins and the expression of the two proteins were analyzed. Results The positive expression rate of Klotho in gastric cancer tissues was 41.2% （28/68） was lower than that of para carcinoma tissues （ 67.5%, P 〈 0. 05 ）, and the correlation was correlated with tumor size, differentiation degree and TNM stage （ P 〈 0. 05 ）. The positive expression rate of LC3 in gastric cancer tissues was 45.6% （31/68） was lower than that in para carcinoma tissues （70. 0% , P 〈 0. 05 ）, and its correlation with tumor size, differentiation degree and TNM stage （ P 〈 0. 05 ）. The expression of Klotho and LC3 in gastric cancer tissues was positively correlated （P 〈 0. 05 ）. Conclusion Autophagy activity in gastric cancer tissue was decreased. The expression of Klotho and LC3 may be involved in the development of gas- tric cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第2期321-323,共3页
Chinese Journal of Experimental Surgery
关键词
KLOTHO
微管相关蛋白1轻链3
胃癌
自噬
Klotho
Microtubule- associated protein 1 light chain 3
Gastric cancer
Autophagy
