摘要
目的观察丙泊酚（propofol，P）对大鼠急性心肌缺血／再灌注（ischemia／reperfusion，I／R）损伤中微管相关蛋白1轻链3（micmtubule-associated protein 1 light3，LC3）的影响，探讨P心肌保护的可能机制。方法采用大鼠在体心肌I／R损伤模型，84只雄性sD大鼠采用随机数字表法分为6组：假手术组（Sham组）、I／R组、脂肪乳组（Int组）、P低剂量组（P1组）、P中剂量组（P2组）、P高剂量组（P3组）。除Sham组外，其余各组均缺血30min，再灌注2h。Int、P1、P2、P3组分别依次经股静脉输注脂肪乳2．4ml·kg^-1·h^-1、P6、12、24mg·kg^-1·h^-1。连续记录功能学指标；2，3，5氯化三苯基四氮唑（2，3，5-trigIhenyhetrazoliumchloride，TYC）染色法检测心肌梗死面积，酶标仪法测定乳酸脱氢酶（1actatedehydrogenase，LDH）的活性，Westemblot分析LC3、磷酸化蛋白激酶B（p-Akt）和蛋白激酶B（Akt）的表达水平。结果与YR组比较，P1、P2、P3组心功能明显改善，心肌梗死面积[（33．8±2．7）％、（27．1±1．9）％和（36．2±312）％VS（50．3±4．2）％，P〈O．05]和血浆LDH水平都显著降低，心肌损伤减轻，LC3II／LC3I比率[（0．97±0．03）、（0．76±0．08）和（0．93±0．04）VS（1．15±0．07），P〈0．05]减低，P-Akt表达[（1．69±0．43）、（2．51±0．15）和（1．95±0．41）vs（1．00±0．13），P〈0．05]增加，而Int组则差异无统计学意义（P〉0．05）；与P1组比较，P2组心功能明显改善，心肌梗死面积[（27．1±1．9）％VS（33．8±2．7）％，P〈0．05]和血浆LDH水平均显著降低，心肌损伤更轻，LC311／LC3I比率[（0．76±0．08）vs（0．97±0．03），P〈0．05]减低，p-Akt表达[（2．51±0．15）vs（1．69±0．43），P〈0．05]增加，而P3组差异没有统计学意义（P〉0．05）。结论P通过抑制LC3的表达，对心肌发挥保护作用，且可能与磷脂酰肌醇-3激酶�
Objective To study the effect of propofol（P） on the expression of microtubule-associated protein 1 light 3（LC3） following acute myocardial ischemia/reperfusion （I/R）injury of rats, and the possible mechanism of P for myocardial protection. Methods The in vivo rat myocardial I/R injury model was developed. Eighty-four male rats were randomly divided into Sham group （Sham）, I/R group, intralipid group （Int）, low-dose P group （P1）, middle-dose P group （P2）, and high-dose P group （P3）. Each group underwent 30 min ischemia and 2 h reperfusion except Sham group. Intralipid 2.4 ml·kg^-1·h^-1 and P 6, 12, 24 mg·kg^-1·h^-1 were infused iv in the last four groups separately. Hemodynamic parameters were monitored continuously. Myocardial infarct size and lactate dehydrogenase （LDH） levels were examined by 2,3,5-triphenyltetrazolium chloride （TIC） staining and enzyme standard method, respectively. Expressions of LC3, p-Akt and Akt were determined by Western blot. Results Compared with I/R group, cardiac function was improved （P〈0.05） in P1, P2 and P3 groups. Myocardial infarct size[（33.8＋2.7）%, （27.1±1.9）% and （36.2± 3.2）% vs （50.3±4.2）%, P〈0.05], the LDH levels in plasma and ratio of LC3 II/LC3 I [（0.97±0.03）, （0,76±0.08） and （0.93±0.04） vs （1.15±0.07）, P〈0.05 ] were decreased respectively in the three groups, while the expression of p-Akt[ （1.69±0.43）, （2.51 ±0.15 ） and （1.95±0.41） vs （1.00±0.13）, P〈0.05] was increased. There was no difference between I/R and Int group （P〉0.05）. Compared with P1 group, cardiac function was significantly improved （P〈0.05）, the expression of p-Akt[ （2.51±0.15 ） vs （ 1.69±0.43 ） ,P〈0.05 ] was increased, with the myocardial infarct size [ （27.1±1.9）% vs （33.8±2.7）%, P〈0.05], LDH levels in plasma and ratio of LC3 11/LC3 II（0.76±0.08） vs （0.97±0.03）, P〈0.053 significantly lower than that of P1 group, while there was no difference in
出处
《国际麻醉学与复苏杂志》
CAS
2014年第3期198-202,共5页
International Journal of Anesthesiology and Resuscitation
关键词
丙泊酚
心肌
再灌注损伤
自噬
微管相关蛋白1轻链3
磷脂酰肌醇-3-激酶
Propofol
Myocardium
Reperfusion injury
Autophagy
Microtubule-associated protein 1 light chain 3
Phosphatidylinositol 3-kinase
