摘要
目的探索准确定位灵长类动物恒河猴大脑深部核团的方法,并以伏隔核作为靶点建立脑深部电刺激恒河猴模型。方法4只恒河猴头颅通过自制适配器固定于CRW-FN立体定位头架,行1.0T磁共振薄层扫描,参考恒河猴脑磁共振和病理对照图谱确定双侧伏隔核位置并计算其坐标,植入脑深部电刺激(deep brain stim-ulation,DBS)电极,利用耳脑胶及内固定钛网固定电极于骨窗处,余部分置于项背部皮下;术后1周,行1.0T磁共振薄层扫描,观察电极位置,由放射科医师测量8个DBS电极末端与同侧伏隔核预定靶点的距离。结果设计出准确的可调节恒河猴脑深部核团定位方法,使4只恒河猴均成功植入双侧电极,术后磁共振复查示电极颅内部分无弯曲、移位,电极末端与伏隔核中心前后方向差距为0.64±0.28 mm,左右方向差距为0.34±0.17 mm。结论通过自制适配器,可以将临床MRI引导有框架立体定向手术系统用于恒河猴,实现个性化、准确的脑深部核团定位和DBS植入。
Objective To explore a method of orientating primates Rhesus＇ deep brain nucleus and to establish a Rhesus model of deep brain stimulation taken Accumbens as the target.Methods Skulls of four rhesus were fixed on CRW-FN stereotactic head frame by self-made adapter,1.0T thin slice MRI scan was performed,locations were orientated and coordinates of the bilateral accumbency were calculated by MRI and pathological atlas of rhesus,then electrodes were implanted,the electrodes were fixed on bone window with Glue and internal fixation titanium mesh,and the rest of the electrodes were planted at the subcutaneousness of neck and back;After 1 week,1.0T thin slice MRI scan were performed,positions of the electrodes were observed,distance between the ends of 8 DBS electrodes and the the intended target of the ipsilateral accumbens was measured by a radiologist.Results A method had been designed to accurately orientate deep brain nucleus of rhesus,four Rhesus were successfully implanted with bilateral electrodes,no bending and shift was showed on postoperative MRI,the distance between the ends of electrodes and the accumbens was 0.64±0.28 mm in the before and after direction,and distance in the left and right direction was 0.34±0.17mm.Conclusion By using self-made adapter,clinical MRI guided stereotactic surgery system can be used on Rhesus to achieve individual and accurate orientation of deep brain nuclei and DBS implantation.
出处
《立体定向和功能性神经外科杂志》
2010年第1期4-7,共4页
Chinese Journal of Stereotactic and Functional Neurosurgery
基金
国家自然科学基金项目(编号:30772219)
关键词
立体定向技术
脑深部核团
伏隔核
恒河猴
脑深部电刺激
Stereotaxic techniques
Deep brain nucleus
Nucleus accumbens
Rhesus monkey
Deep brain stimulation
