摘要
氨基酸是生命有机体的重要组成部分,参与人体各项生理活动和新陈代谢,在人体中发挥着特殊的作用,是人体必不可少的营养成分之一。近年来,生命科学的研究与食品、药品和保健品的开发,以及麻醉药品、精神药品对兴奋性神经递质的影响的研究,促进了对存在于各种检验材料中的氨基酸的分析方法的研究,探索高灵敏度和高选择性的氨基酸分析检测方法引起了人们极大的兴趣,所以建立快速、准确的氨基酸的检测方法越来越重要。毛细管电泳(capillary electrophoresis,CE)是继高效液相色谱之后发展起来的一种新的分离和分析方法,具有分离效率高、分析速度快、样品及试剂用量少等特点,本文参考近几年的相关文献,对氨基酸进行毛细管电泳检测使用紫外检测器、荧光检测器、电化学检测器和质谱检测器的方法作一综述。
Amino acids are an important part of life,which take part in physical activity and metabolic of human and are essential nutrients of body.In recent years,life science research,food,medicines and the development of health products,as well as the study of narcotic drugs,psychotropic drugs to excitatory neurotransmitter,that promote the study of amino acid analysis in all kinds of materials,and explore high sensitivity and high selectivity of amino acid detection method has aroused great interest,so It is important to develop rapie and accurate methods to determine amino acids.Capillary electrophoresis（CE） is a new separation and analysis methods after the high-performance liquid chromatography,that has the characteristics of high separation efficiency,rapid analysis and less samples.In this paper,the methods of amino acids detection by capillary electrophoresis using UV detector,fluorescence detector,electrochemical detector and mass spectrometry detector are reviewed.
出处
《现代生物医学进展》
CAS
2010年第1期194-196,200,共4页
Progress in Modern Biomedicine
基金
云南省科技厅应用基础研究面上项目(2008ZC114M)
关键词
氨基酸
毛细管电泳
检测器
Amino acids Capillary electrophoresis Detector
