摘要
目的探讨Foxp3+淋巴细胞在人乳腺癌组织中的表达及其与其他临床病理因素的关系,并对其可能作用机制进行探讨。方法采用流式细胞术(FCM)检测30例乳腺癌患者肿瘤组织及癌旁正常组织中Foxp3+淋巴细胞百分率,并采用多元线性回归分析法统计分析肿瘤组织中Foxp3+淋巴细胞表达与患者临床病理学特征的相互关系;免疫组化法检测肿瘤组织及癌旁正常组织中浸润淋巴细胞中TGF-β和IL-10表达情况,并采用直线相关分析法统计分析其与Foxp3+淋巴细胞表达的相关性。结果肿瘤组织中Foxp3+淋巴细胞百分率明显高于癌旁正常组织(P<0.05),同时肿瘤组织中Foxp3+淋巴细胞百分率与临床分期、有无脉管瘤栓、腋淋巴结转移等临床病理因素有关(P<0.05)。肿瘤组织浸润淋巴细胞的IL-10表达与肿瘤组织中Foxp3+淋巴细胞百分率正相关(P<0.05)。结论Foxp3+淋巴细胞与乳腺癌侵袭转移有关,其机制与其分泌IL-10抑制机体抗肿瘤免疫有关。Foxp3+淋巴细胞可作为乳腺癌预后判断的辅助指标,也有望成为乳腺癌临床治疗的新标靶。
Objective To investigate the expression of Foxp3＋ lymphocytes in breast carcinoma tissues and their correlation with other pathological factors,and to investigate the mechanism of action of Treg cells.Methods The expression of Foxp3＋ lymphocytes in the breast cancer tissue and non-cancerous tissue was detected by flow cytometry （FCM） in 30 breast carcinoma patients,and its correlation with other pathological factors was statistically analyzed by multiple linear regression analysis.The expression of TGF-β and IL-10 in the lymphocytes infiltrated in breast cancer tissue and non-cancerous tissue was measured by immunohistochemistry,and their correlation with the expression of Foxp3＋ lymphocytes was statistically analyzed by linear correlation dependability analysis.Results There was significant difference in the expression of Foxp3＋ lymphocytes between the malignant and non-cancerous breast tissues（P0.05）,and it was positively correlated with the clinical stage,blood vessel invasion and the matter of axillary lymph node metastasis（P0.05）.The expression of IL-10 in the tumor infiltrating lymphocytes was positively correlated with the expression of Foxp3＋ lymphocytes（P0.05）.Conclusion The expression level of Foxp3＋ lymphocytes is correlated with invasion and metastasis of breast carcinoma,and the IL-10 secreted by Foxp3＋ lymphocytes may be involved in this effect.Foxp3＋ lymphocytes can be used as an assistant marker for prediction and new therpeutic target of breast cancer.
出处
《军事医学科学院院刊》
CSCD
北大核心
2010年第1期61-64,67,共5页
Bulletin of the Academy of Military Medical Sciences
基金
河北普通高等学校强势特色学科建设课题项目
