摘要
目的探讨生长素释放肽（Ghrelin）对盲肠结扎穿孔（CLP）介导的急性肺损伤小鼠早期核因子xB（NF-κB）和纤溶系统的影响。方法将32只昆明小鼠随机分为正常组、假手术组、CLP组和Ghrelin干预组，Ghrelin干预组在ClP后5h、10h、15h时间段分次腹腔注射Ghrelin（共40nmol／kg）。采用HE染色进行病理检测，免疫组织化学方法检测NF-κB表达，采用酶联免疫吸附试验方法检测血浆纤溶酶原激活物抑制因子-1（PAI-1）、组织型纤溶酶原激活物（t—PA）水平，计算t—PA／PAI-1比值。结果①病理结果显示：与正常组、假手术组相比，CLP组部分肺泡结构受到破坏，大量炎症细胞浸润，间质水肿明显，部分有出血，Ghrelin干预组肺组织病理损伤程度明显减轻。②免疫组织化学结果显示：与正常组、假手术组相比，CLP组肺组织NF—KB表达显著增强，Ghrelin干预组的NF—κB表达强度比CLP组弱。③与正常组、假手术组相比，CLP组PAI-1、t—PA水平明显升高（P〈0．01），t-PA／PAI-1比值明显下降（P〈0．01）。Ghrelin干预组的血浆PAI-1水平较CI，P组明显下降（P〈0．01），血浆t-PA水平较CLP组轻度升高（P〈0．05），t-PA／PAI-1比值较CLP组明显升高（P〈0．01）。结论急性肺损伤小鼠早期纤溶系统功能抑制，Ghrelin可以降低CLP所致肺组织NF-κB的表达，Ghrelin可能通过NF—κB途径减轻CLP介导的急性肺损伤，提高纤溶活性。
Objective To investigate the effect of ghrelin on nuclear factor-κB （NF-κB） and fibrinolytic system i.n acute lung injury mice induced by cecal ligation and perforation （CLP） at early stage. Methods Thirty-two Kunming mice were randomly divided into normal group, sham-operated group, CLP group and ghrelin intervention group. Ghrelin intervention group received intraperitoneal injection with ghrelin at 5 h,10 h,15 h time points after CLP （total 40 nmot/kg）. HE staining was used for pathological detection. The expression of NF-κB was detected by immunohistochemistry. Plasma plasminogen activator inhibitor 1 （PAI 1） and tissue-type plasminogen activator （t-PA） were detected by enzyme linked immunosorbent assay, and the ratio of t-PA/PAI-1 was computed. Results ①Pathology results showed that compared with normal group and sham-operated group, part of alveolar cell structure was damaged,a great deal of inflammatory cell infiltrated, pulmonary interstitial edema was obvious, part had haemorrhage in CLP group. The pathologic＇damage of pulmonary tissue reduced significantly in ghrelin intervention group, ②Immunohistochemistry results showed that compared with normal group and sham-operated group, NF-κB expression of pulmonary tissue markedly increased in CLP group. The expression of NF-κB in ghrelin intervention group was weaker than that in CLP group. QCompared with normal group and sham-operated group, plasma PAI-1 and t-PA level increased significantly （ P 〈0.01 ）, t-PA/PAI-1 obviously decreased （ P 〈0.01） in CLP group. Compared with CLP group, plasma PAI-1 significantly decreased （ P 〈0. 001 ）, plasma t PA slightly increased （ P 〈 0.05）, and t-PA/PAI -1 significantly increased（ P 〈0.01） in CI.P group. Conclusions The fibrinolytic system is inhibited at the early stage of acute lung injury in mice. Ghrelin can reduce the expression of NF-κB in pulmonary tissue caused by CLP. Ghrelin may relieve acute lung injury induced by CLP and enhance fibrinolytic activ
出处
《国际呼吸杂志》
2010年第6期333-336,共4页
International Journal of Respiration
