摘要
目的:观察急性和慢性再障患者联合免疫疗法治疗前、后外周血和骨髓中白细胞介素8(IL-8)的含量变化和临床意义,并探讨其对监测再障疗效的可行性。方法:应用实时定量PCR和ELISA法检测外周血和骨髓IL-8的含量,流式细胞术分析外周血中CD4/CD8阳性细胞比值变化。结果:急性和慢性再障患者外周血和骨髓中IL-8含量均远高于正常对照者,且CD4/CD8出现倒置。抗淋巴细胞球蛋白(ALG)加环孢素A(CsA)加雄激素较CsA加雄激素治疗效果明显,总有效率分别为83.3%和62.5%。ALG加CsA加雄激素治疗重症再障较CsA加雄激素疗效显著。细胞因子水平改变和疗效明显相关。实时定量PCR和ELISA法正相关,但前者较易测出和量化。结论:ALG联合CsA治疗急、慢性再障疗效显著。细胞因子IL-8可以作为急、慢性再障的疗效监测指标。
Objective：To investigate the alteration of cytokine expression after immunosuppressive therapy, analyze their clinical significance in patients with aplastic anemia（AA）, and explore the possibility of therapeutic efficacy on cytokine IL-8. Method： Amount of IL-8 in peripheral blood and bone marrow was measured by real time PCR methods. Changes of CD4/CD8 positive cells were analyzed by flow cytometery. Result：The level of cytokine IL-8 in peripheral blood and BMP of aplastic anemia patients was significantly increased than that in the normal control, and the ratio of CD4/CD8 reversed. The level of IL-8 in acute AA （type Ⅰ 〉type Ⅱ ） was higher than that in chronic AA. The ALG＋CsA＋ male hormone therapy had been proved to be more effective than CsA＋ male hormone therapy. The total efficacy rate was 82.3% and 69.3%, and recovered CD4/CD8 ratio. The ALG＋CsA ＋male hormone had a better effect in acute AA （type Ⅰ〉type Ⅱ ）. There was a significant correlation between the alteration of IL-8 and CD4/CD8 with therapy efficacy. Real time PCR was positively correlated with ELISA, and more easily detected and quantized. Conclusion：ALG allied CsA is effective in acute and chronic aplastic anemia therapy. Cytokine changes of IL-8 may be used as a serveillance marker in diagnosis and treatment in aplastic anemia.
出处
《临床血液学杂志》
CAS
2010年第1期32-35,共4页
Journal of Clinical Hematology
