摘要
目的探讨北方地区汉族人干扰素(IFN-r)基因单核苷酸多态性(SNP)和IFN-r血中表达水平与急性早幼粒细胞白血病(APL)的相关性。方法应用顺序特异性引物和聚合酶链反应(PCR-SSP)技术,检测了21例APL患者(APL组)和83例健康献血者(对照组)中IFN-r启动子基因874位点的多态性变化,同时应用酶联免疫吸附试验(ELISA)测定两组血清中IFN-r的表达水平。结果结果表明APL患者A/A基因型频率明显著低于健康对照组(χ2=3.88,OR=1.68,P<0.05),A/T和T/T基因型频率以及A和T等位基因频率差异无统计学意义(P>0.05);IFN-r含量APL组(1.011±0.108)和对照组(0.721±0.065),两组之间比较差异无显著性意义,提示A/A基因型与APL相关。结论我国北方地区汉族人IFN-r基因874位点的多态性在APL的发病中具有重要作用,IFN-r启动子基因A/A基因型可能是急性早幼粒细胞白血病拮抗性基因之一。
Objective; To investigate the relationship between the levels of Interferon IFN - r） , IFN - r gene polymorphism and acute promyelocyti leukemia. Methods： The level of plasma IFN - r was measured by ELISA, and PCR - SSP was used to detect the polymorphism of IFN - r 874T and 874A gene. Results： Compared with APL, healthy subjects had a great frequency of A/A genotype at IFN- r 874 gene promoter position （ χ2 = 3.88, OR = 1.68, P 〈 0. 05 ）, the frequencies of 874 A/T and T/T genotype were not significantly different. Frequencies of IFN - r 874T and A were not significantly higher than those in healthy controls （ P 〉 0. 05 ）. Level of IFN - r in study group （ 1.011 ± 0. 108 ） μg/L was not significantly than the control group （0. 721 ± 0. 065 ） ） μg/L, P 〉 0. 05. Conclusion： Our results indicate that the change of genie polymorphism of cytokine is correlated with APL. IFN -r （ ＋ 874T） A/A genotype genotype were significantly related to patients with APL. IFN-r （＋874T） A/A genotype can have some function resistant to APL.
出处
《中国优生与遗传杂志》
2008年第2期39-40,61,共3页
Chinese Journal of Birth Health & Heredity
