摘要
【目的】大麦Wx是控制直连淀粉合成相关的糯性基因,研究大麦糯性相关基因Wx单核苷酸多态性,并分析其与籽粒直链淀粉含量的关系。【方法】以2个国外糯大麦品种为对照,对30份高、中、低直链淀粉含量的中国大麦进行Wx的克隆和测序,分析Wx的单核苷酸多态性(SNP)及其与籽粒直链淀粉含量之间的关系。【结果】在对32个大麦品种的核苷酸序列多态性鉴定中,共检测到了169个多态性位点,平均每26bp检测到一个多态性位点。在所有检测到的多态性位点中,包括143个SNP和26个InDel,二者的频率分别为1/310和1/169。Wx的内含子1、3、5、8区,外显子2、5和5′-UTR及3′-UTR区域为变异富集区,其它区域变异较小。外显子2和内含子1区域所承受的选择压力较小。单倍型分析表明,第1种单倍型中包括所有低直链淀粉含量的材料。【结论】大麦Wx的多态性与直链淀粉含量之间存在明显的对应关系。
[ Objective] Waxy gene （Wx in short） is a key gene responsible for the barley amylose synthesis. The objective of the present study was to detect single nucleotide polymorphisms （SNP） of Wx and determine the relationship between SNP and amylose content of seed in barley. [Method] After PCR amplification and gene clone, 30 Chinese normal and waxy barley cultivars, which had high, medium and low amylose content respectively, were＇, sequenced and polymorphism was analyzed for waxy gene with 2 Canadian waxy barley as controls. [Result] By polymorphism identification of waxy gene nucleotide sequences for the 32 barley cultivars, 169 polymorphism sites were identified, which included 143 SNP and 26 InDel with frequencies of 1/310 and 1/196, respectively. It was found that the introns 1,3,5,8, exons 2,5 and 5＇-UTR, 3＇-UTR were the rich nucleotide variation regions. Exon 2 and intron 1 suffered stronger selection pressure. Four haplotypes were identified from 32 plant materials. Among them, haplotype 1 included all low amylose content accessions. [ Conclusion] The results of research showed that there is a clear correlation between SNPs in waxy gene and amylose content in barley.
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第5期889-898,共10页
Scientia Agricultura Sinica
基金
农业部引进国际先进农业科学技术“948”项目(2006-G9)
农业部公益行业科研项目(nyhyzx07-010)
