摘要
玉米淀粉合成酶Zm SSIIa在淀粉生物合成途径中主要作用是将支链淀粉的分支链由6~10个延长至12~25个,对于玉米籽粒产量和品质具有重要的影响。本研究在86个优异玉米自交系中对该基因进行基因序列定点捕获,并获得该基因全长为4836 bp的核苷酸序列。基因多态性分析发现,该基因座位上有78个SNP和19个Indel。玉米Zm SSIIa基因的编码区具有24个SNP,将该基因划分成9种编码区单倍型,并编码7种Zm SSIIa蛋白质。供试材料的群体中,Zm SSIIa基因至少经历8次重组,可能对单倍型的分化和连锁不平衡具有重要作用。中性进化测试表明该基因在供试群体中没有明显的人工选择。
The maize starch synthesis ZmSSIIa elongates amylopectin chains from degree of polymerization 6-10 to degree of polymerization 12-25 in the pathway of starch biosynthesis, and plays important roles in kernel yield and quality. The analysis of sequence polymorphism of maize ZmSSIIa gene will provide information for further detection of beneficial mutants associated with yield and quality. In order to reveal the sequence polymorphism of the maize ZmSSIIa gene, a total of 4836 bp sequence of this locus is captured in 86 elite maize inbred lines through a NimblcGensequence capture array. Analysis of the sequence polymorphism reveals that there are ＂78 SNPs and 19 lndels in this locus. The coding sequences of the maize ZmSSIIa gene contain 24 SNPs, which divide the coding regions into 9 haploytpes, and 7 different ZmSSIIa proteins are encoded by the tested inbred lines. At least 8 recombination events are found to contribute to the haplotype diversity and linkage disequilibrium between polymorphic sites in the maize ZmSSIIa gene. According to the results of the test of neutral evolution, there is no evidence of artificial selection for this gene in the tested inbred lines.
出处
《科技导报》
CAS
CSCD
北大核心
2014年第36期52-58,共7页
Science & Technology Review
基金
高等学校博士学科点专项科研基金项目(20123250110001)
江苏省自然科学基金项目(BK2012261)
江苏省高校自然科学研究重大项目(14KJA210005)
江苏省"青蓝工程"科技创新团队项目
