摘要
采用聚合酶链式反应 (PCR)技术对粤西镇海湾水域的近江牡蛎Crassostrearivularis(Gould)群体 2 7个个体的线粒体DNA16SrRNA基因序列片段进行扩增 ,获得大约 5 0 0bp的扩增产物。PCR产物经纯化后进行序列测定 ,经ClustalX同源排序 ,除去引物及部分端部序列 ,得到4 14bp的核苷酸片段。 2 7个个体共检测到 2个变异位点 ,均为颠换位点 ,没发现碱基位点插入、缺失及转换位点 ,共 3种单倍型 ,每个单倍型只有一个碱基的差异。运用DNASP软件计算得该群体的核苷酸多样性和平均核苷酸差异数分别为 0 .0 0 0 36和 0 .14 815。此结果提示镇海湾近江牡蛎群体遗传多样性已很低 。
The PCR technique was used to amplify the mtDNA 16S rRNA gene in 27 individuals of southern oyster (Crassostrea rivularis) from Zhenhai Bay. The PCR products were purified and sequenced. As a result, 414 bp nucleotide sequences of partial 16S rRNA gene were obtained (the primer and some of the marginal sequences were excluded). By using Clustal X to align and compare the sequences of these 27 individuals with each other, 2 variation sites were observed , and the variation sites were transversion sites, there was 3 haploty in total. As estimated by DNASP, the number of polymorphic sites (S) is 2; the nucleotide diversity (P i) and the average number of nucleotide differences (K) are 0.000 36 and 0.148 15, respectively. The results show that the genetic diversity of Crassostrea rivularis from Zhenghai Bay is exceptionally low, so it is necessary to introduce the parents of Crassostrea rivularis from other areas.
出处
《湛江海洋大学学报》
2004年第4期1-5,共5页
Journal of Zhanjiang Ocean University
基金
广东省科技计划项目 2 0 0 2B2 15 0 10 2
国家高技术研究发展计划项目 2 0 0 1AA6 2 70 30资助
