摘要
建立了基质固相分散萃取(matrix solid phase dispersion,MSPD)-反相高效液相色谱(reversed phase high performance liquid chromatography,RP-HPLC)检测利谷隆、灭草隆、敌草隆和绿麦隆4种苯脲类除草剂的新方法。茶叶样品与C18填料混合碾磨后与中性氧化铝一起装柱,二氯甲烷为淋洗剂,使样品的萃取与净化一步完成。讨论了MSPD法和色谱分析的各种影响因素,最佳实验条件下灭草隆在0.01～10.00 mg/L范围内线性关系良好,检测限为5μg/L;绿麦隆、敌草隆和利谷隆在0.025～10.00 mg/L范围内线性关系良好,检测限为10μg/L。4种除草剂在茶叶样品中的回收率在83.5%～100.4%之间,相对标准偏差小于7.1%。该方法简单、快速、干扰少、回收率高,符合茶叶中除草剂残留分析要求。
A new method for the simultaneous determination of 4 herbicides （Monuron, Chlortoluron, Diuron and Linuron） was established with MSPD-RP-HPLC. The mixture of tea samples and C18 stuffing were pulverized, then mixed with alumina packed to a syringe column, the extraction and purification of the samples were carried out with one-step using dichloromethane as eluting reagent. The effecting factors of MSPD and conditions of the chromatography were discussed. Under optimum conditions, the linear ranges of Monuron was 0.01-10.0 mg/L with 5 μg/L of the detection limits, and the linear ranges of Chlortoluron, Diuron and Linuron were 0.025-10.0 mg/L with 10 μg/L of the detection limits. The average recoveries of 4 herbicides ranged from 83.5% to100.4% with less than 7.1% of the relative standard deviations in tea samples. The method suggested was simple, rapid, and less interference with high recovery rate, and meet the analytical requirements of herbicide residues in tea.
出处
《茶叶科学》
CAS
CSCD
北大核心
2010年第1期52-56,共5页
Journal of Tea Science
基金
浙江省科技厅大型仪器测试基金(2007F70051)
