摘要
为分析48份糯玉米自交系的遗传多样性及其之间的遗传关系，利用总DNA提取方法和微卫星分子标记（SSR）技术，取均匀分布各染色体的45对SSR引物对样品进行PCR扩增分析，筛选出23对扩增带型稳定的SSR引物，从供试材料中检测出141个等位基因变异，每对引物检测等位基因2～14个，平均5．48个。SSR引物的PIC介于0．43～0．89，平均多态性信息量为0．66。UPGMA方法聚类分析表明，供试材料间遗传距离变幅为0．26～0．72，平均值为0．49。SSR标记能将全部自交系区分开来，48份糯玉米自交系可分为六类。
The genetic diversity of 48 glutinous maize inbred lines and the genetic relationship between these lines were analyzed by the SSR molecular marker and total DNA extraction method. 23 pairs of stable SSR primers with the amplification band pattern were amplified from 45 pairs of SSR primers of samples. 141 alleles were detected from 48 tested inbred lines and 2-4 alleles with average 5.48 alleles were detected from a pair of primer. The polymorphism information content and mean polymorphism information content of each SSR primer was 0. 43-0. 89 and 0. 66. The results from UPGMA clustering analysis showed that the genetic distance between tested lines was 0. 26 -0. 72 and its mean value was 0.49.48 The glutinous maize inbred lines can be divided into six groups, which can provide a reference for its breeding.
出处
《贵州农业科学》
CAS
北大核心
2010年第1期1-4,共4页
Guizhou Agricultural Sciences
基金
国家科技支撑计划项目"喀斯特山区特有作物优异基因资源发掘与转基因安全关键技术研究与应用"(2007BAD59B00)
关键词
糯玉米
SSR标记
遗传多样性
聚类分析
glutinous maize
SSR marker
genetic diversity
clustering analysis
