摘要
利用SSR分子标记构建了2009-2010年黄河流域23个主要棉花品种和2010年山东省区试4个品系的分子指纹图谱并进行遗传多样性分析。63对引物在27份供试材料中共扩增到多态性条带254条,平均每对引物4.03条。22对引物在23个品种(系)上具有特征谱带,除鲁棉研21号、鲁棉研36号、鲁436、邯棉559外,均可用1对引物进行鉴定;采用引物组合法只需要5对引物就能将27份供试材料区分开,并利用这5对引物构建了上述品种(系)的数字指纹图谱。遗传多样性分析显示,27个品种(系)间遗传相似系数介于0.5394～0.9685之间,平均为0.6878,并且同一省份所选育的品种(系)大都聚到同一类群,说明黄河流域选育的品种还存在一定的地域局限性。
SSR markers were used to analyze the fingerprinting and genetic diversity of 23 major cultivars in the Yellow River Valley region during 2009-- 2010 and four varieties in Shandong provincial regional trial in 2010. Among 381 pairs of primers, 63 pairs with high polymorphisms and good repeatability were selected. Totally, 254 polymorphic bands were detected, with an average of 4.03 bands per primer. 23 varieties had unique bands by 22 primer pairs, which could be identified with one pair of specific primer except Lumianyan 21, Lumianyan 36, Lu 436 and Hanmian 559. All varieties could be distinguished by five primer combinations at least, and digital fingerprinting code was also established. UPGMA cluster analysis was used to estimate the genetic distances and to construct a dendrogram by NTSYS 2.1. The results showed that the coefficient of genetic similarity range was 0.5394-0.9685, with an average of 0.6878. Most of the varieties derived from the same province were clustered together, indicating their limitations on the geographic area.
出处
《棉花学报》
CSCD
北大核心
2011年第6期545-551,共7页
Cotton Science
基金
国家转基因生物重大专项(2008ZX08005-001)
山东省农业良种工程(2010LZ005-02)
关键词
棉花
黄河流域
品种
SSR指纹图谱
遗传多样性
cotton
Yellow River Valley
major varieties
SSR fingerprinting
genetic diversity
