摘要
建立了检测氯胺酮的生物素-亲和素放大酶联免疫吸附测定法(BA-ELISA)。实验最佳测定条件:抗原包被浓度为2.0mg/L、氯胺酮单克隆抗体浓度为10.2mg/L,生物素化羊抗小鼠IgG(Biotin-IgG)和酶标链霉亲和素(SA-HRP)的最佳反应浓度分别为0.29和1.0mg/L。在此优化条件下,方法的线性范围为0.1～1000μg/L;检出限为0.03μg/L。氯胺酮生物样品的加标回收率为94%～102%。与酶标二抗体系ELISA法相比,BA-ELISA具有更高的灵敏度,适于低浓度氯胺酮的检测。
A rapid and sensitive method based on biotin-avidin mediated competitive enzyme-linked immunosorbent assay（BA-ELISA） was established for the determination of ketamine.The optimal concentration of coated antigen and anti-ketamine monoclonal antibody were found to be 2.0 and 10.2 mg/L.The concentrations of biotinylated goat anti-mouse IgG（Biotin-IgG） and streptavidin-horseradish peroxidase（SA-HRP） were optimized and the optimum results were found to be 0.29 and 1.0 mg/L,respectively.The linear range of the presented method was from 0.1 to 1000 μg/L,and the limit of detection was found to be 0.03 μg/L.The recoveries of ketamine spiked in human serum and urine were between 94% and 102%.Comparing the result of traditional ELISA,the present BA-ELISA method had a lower detection limit for ketamine.The experimental results indicated that the present BA-ELISA method was specific and sensitive.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第1期117-120,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.90713013)资助项目
关键词
氯胺酮
单克隆抗体
生物素-亲和素放大酶联免疫吸附法
Ketamine
Monoclonal antibody
Biotin-avidin mediated competitive enzyme-linked immunosorbent assay
