摘要
目的:观察中药复方肌力康饮对实验性自身免疫性重症肌无力(EAMG)大鼠血清AchR-Ab、TGF-β1水平的影响,以探讨其治疗重症肌无力的可能机制。方法:60只Lewis大鼠随机分为6组,每组10只:空白组,模型组,阳性药物组,肌力康饮高、中、低剂量组。采用AchR主动免疫的方法免疫除空白组外的各组大鼠,制备EAMG大鼠模型。确立模型成功,各组给药治疗4周后,采用ELISA法检测血清AchR-Ab、TGF-β1水平。结果:肌力康饮各组、阳性药物组能明显改善EAMG大鼠肌无力症状,临床症状评分显著降低、血清AchR-Ab水平明显下降、TGF-β1水平明显升高,与模型组比较,有显著性差异(P<0.01);肌力康饮高剂量组与阳性药物组比较亦有明显差异(P<0.05);但肌力康饮各剂量组之间比较无显著性差异(P>0.05)。结论:肌力康饮下调了EAMG大鼠过高的AchR-Ab水平,并使TGF-β1升高,从而改善了EAMG大鼠的临床症状。
Objective： Observe the effect of Ji Li Kang Drink on experimental autoimmune myasthenia gravis （EAMG） serum AchR - Ab, TGF - β1 levels in order to explore the possible mechanisms of treatment of myasthenia gravis. Methods： 60 Lewis rats were randomly divided into six groups of 10： blank group, model group, positive medicine group, high - dose group of Ji Li Kang drink , medium - dose group and low - dose group. Method of active immunization with AchR was used to prepare for EAMG rats. After being established a successful model, each group were detected serum AchR - Ab, TGF - 131 levels using ELISA after 4 weeks drug treatment. Results ： groups of Ji Li Kang drink and positive medicine group can significantly improve the symptoms of EAMG rats;Score of clinical symptom significantly decreased ; AchR - Ab levels in serum were significantly decreased ; TGF - β1 levels were significantly elevated compared with model group. There was significant difference （ P 〈 0.01 ）. Groups of Ji Li Kang drink and the positive medicine group also were significantly different （ P 〈 0.05 ） ; Each dose group of Ji Li Kang drink showed no significant difference （ P 〉 0. 05 ）. Conclusion： Ji Li Kang drink reduced AchR - Ab level , and increased TGF - β1 level of EAMG rats Thus Ji Li Kang drink improved the clinical symptoms of EAMG rats.
出处
《中华中医药学刊》
CAS
2010年第1期80-82,共3页
Chinese Archives of Traditional Chinese Medicine
基金
黑龙江省自然基金资助项目(D2007-65)
