摘要
目的探讨血管内皮生长因子(VEGF)及其受体KDR在血管瘤中的表达以及其与微血管密度(MVD)的关系。方法采用免疫组化SP法检测35例婴幼儿血管瘤VEGF及KDR的表达和MVD。结果35例血管瘤中VEGF、KDR的蛋白表达阳性率分别是66.5%,73.4%。其中增生期和消退期VEGF阳性率分别为92.1%、31.4%(P<0.01),KDR阳性率分别为89.3%、42.5%(P<0.01);MVD分别为75.7±13.71、28.9±8.5(P<0.01)。KDR的表达与VEGF成正相关(P<0.01),KDR和VEGF与MVD都成正相关(P<0.01)。结论血管瘤增生期血管内皮细胞存在着血管内皮细胞生长因子及其受体KDR的相互作用,促进了新生微血管生成。
Objective To investigate the relationship between the expression of the vascular endothelial cell growth factor（VEGF） and KDR and mierovessel density（MVD） in hemangioma. Methods lmmunohistochemical streptavidin/peroxidase（SP） stain was applied to examine the expression of KDR, VEGF and CD34. The amount of microvessel was counted by the expression CD34 in endothelial cell. Results In 35 cases, positive rate for KDR was 73.4% ,for VEGF was 66. 5%. In the proliferating phase,positive rate for KDR was 89. 3% ,for VEGF was 92. 1%. However, the positive rate for KDR,in the involuting phase,was 42. 5%, and for VEGF was 31.4%. There was a very significantly difference between two phases（P 〈0. 01 ）. In the proliferating phase,MVD was （75.7 ± 13.71 ）. In the involuting phase, MVD was （ 28. 9 ± 8.5 ） （ P 〈 0. 01 ）. The correlation between the KDR and VEGF was positive （ P 〈 0. 01 ）. There was a positive correlation between the KDR and MVD （ P 〈 0. 01 ）. Conclusion There might be a speeial interaction between the KDR and VEGF in endothelial cell of the hemangioma in proliferating phase, which could result in the angiogenesis.
出处
《中国临床新医学》
2009年第11期1131-1134,共4页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
广东省湛江市科技攻关项目(编号:2008C02004)
