摘要
目的比较纤维素酶、半仿生、乙醇回流提取方法对川乌中乌头碱、乌头总碱的影响,筛选最佳方法。方法利用纤维素酶辅助提取川乌生物碱,以乌头碱、乌头总碱提取率为指标,正交试验优化纤维素酶的最佳提取条件,再与半仿生、乙醇提取方法进行比较。结果提取乌头碱、乌头总碱的最佳酶解条件为:温度45℃,pH值4.5,酶用量8mg/ g,酶解时间5h,乌头碱提取率为0.002 447%,乌头总碱提取率为0.244 410%,半仿生提取乌头碱、乌头总碱的提取率分别为0.001 735%、0.189 340%,乙醇回流提取乌头碱、乌头总碱提取率分别为0.001 869%、0.200 720%,纤维素酶提取法具有明显的优势。结论酶解提取法可显著提高乌头碱、乌头总碱的提取率,可在实际生产中推广应用。
Objective To compare the extraction methods of cellulose enzyme, semi-bionic and alcohol reflux for the impact of aconitine and aconitum alkaloids yields, and to optimize the extraction method. Methods The optimum condition of enzymatic extraction has been obtained by orthogonal test and compared with semi-bionic and alcohol reflux extraction by taking the contents of aconitine and aconitum alka loids as indexes. Results The optimum extraction conditions were as follows： the temperature was 45℃, pH value was 4.5, the amount of cellulose enzyme was 8 mg/g, the extracting time was 5 h and the extraction rate of aconitine was 0. 002 447% , aconitum alkaloids was 0. 244 410%. The extraction rates of aconitine and aconitum alkaloids of semi-bionic extraction were 0. 001 735% and 0. 189 340%, respectively. The extraction rates of aconitine and aconitum alkaloids of alcohol reflux extraction were 0. 001 869% and 0. 200 720% respectively. The enzymatic extraction has a significant advantage. Conclusion The enzymatic extraction significantly improve the extraction rates of aconitine and aconitum alkaloids, it could be applied in practice.
出处
《中草药》
CAS
CSCD
北大核心
2009年第11期1735-1739,共5页
Chinese Traditional and Herbal Drugs
基金
北京市人才强教计划-中青年骨干教师资助项目
关键词
川乌
纤维素酶
乌头碱
乌头总碱
正交设计
Radix Aconiti
cellulose enzyme
aconitine
aconitum alkaloids
orthogonal design
