摘要
蛋白质定向进化技术是蛋白质分子改造的一个重要策略。重点介绍了易错PCR、DNA改组等对编码蛋白质的基因进行随机突变和重组的技术,以及构建突变体库和高通量筛选的方法,并探讨了定向进化技术在蛋白质工程中的应用及前景。
Directed evolution of protein is the effective strategy for the protein improvement. This review introduced the techniques for the mutation and recombination of DNA using error-prone PCR, DNA shuffling methods, and generation of mutational library and high throughput screening. Meanwhile, recent applications and development of directed evolution of protein were also reviewed in this paper.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第11期43-47,共5页
Biotechnology Bulletin
基金
国家"863"计划项目(2007AA100605)
关键词
定向进化
易错PCR
DNA改组
高通量筛选
Directed evolution Error-prone PCR DNA shuffling High-throughput screening
