摘要
B细胞易位基因1(BTG1基因)是BTG/TOB基因家族的成员之一,在动物细胞的增殖和分化中起重要的作用.利用牛BTG1基因的mRNA序列与绵羊的EST数据库进行Blast检索,并通过序列拼接和逆转录RT-PCR方法首次获得绵羊BTG1基因的部分cDNA序列(GenBank登录号FJ444829),其片段长度为1 358 bp,包括完整的开放阅读框516 bp,编码171个氨基酸.半定量PCR研究结果表明:BTG1基因在小尾寒羊和陶赛特羊的10种组织中均表达,并具有一致的表达趋势.同源分析结果表明,绵羊BTG1蛋白的氨基酸序列中存在BTG/TOB的保守结构域,并且该蛋白在不同物种间具有很高的保守性.通过生物信息学预测BTG1蛋白功能,发现绵羊BTG1蛋白存在1个跨膜结构域、8个磷酸化位点和1个特异性蛋白激酶磷酸化位点.蛋白质结构同源建模分析表明,绵羊BTG1蛋白具有BTG/TOB蛋白家族的典型空间结构.
BTG 1 （ B-cell translocation gene 1） is a member of the BTG/TOB gene family, which plays a role in the proliferation and differentiation in animal cells. Based on blast searches with the mRNA sequence of cattle BTG1 and the sheep-EST databases, a 1 358 bp cDNA fragment of BTG1 gene （GenBank accession No. FJ444829 ） was first amplified by RT-PCR. The sheep BTG1 contains a 516 bp ORF encoding 171 amino acids. Semi-quantitative PCR revealed that the BTG1 was similarly expressed in ten types of tissues studied of both Small Tailed Han sheep and Dorset sheep breeds. Homology analysis showed that the amino acid sequences of sheep BTG1 was highly homologous to other species, containing the BTG/TOB conserved domains. By homologous modeling, the predicted structure of BTG1 was similar other members of BTG/TOB with one transmembrane domain, eight phosphorylation sites, including one of specific protein kinase.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2009年第10期911-917,共7页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家科技支撑计划项目-优质专用羊新品种选育(No.2006BAD01A11)~~
