摘要
利用生物信息学软件对Henan-09-03株乙型脑炎病毒(JEV)病毒E蛋白(囊膜蛋白)进行生物信息学分析、预测,为研究JEV的E蛋白基因工程疫苗提供依据。结果表明:E蛋白的分子质量为53 k Da,等电点是7.75,消光系数是66 515,不稳定系数为27.05,疏水平均值为-0.007,无信号肽,有2个跨膜区、27个蛋白修饰位点、5个二硫键、15个蛋白质结合位点,二级结构为α-β混合型、三级结构以同源四聚体形式存在。
Bioinformatics of envelope protein of Japanese encephalitis virus Henan-09-03 strain was analyzed and predicted by information biology software for researching new type genetic engineering vaccine. The results showed that the molecular weight of envelope protein was 53 kDa,isoelectric point was 7. 75,extinction coefficient was 66 515,instability index was 27. 05,grand average of hydropathicity was-0. 007. It had no signal peptide,（ two transmembrane domains,twenty-seven protein modification sites,five disulfide bonds,fifteen protein binding sites.Its secondary structure was alpha-beta mixed type,and tertiary structure was homotetramer.
出处
《华北农学报》
CSCD
北大核心
2015年第5期36-40,共5页
Acta Agriculturae Boreali-Sinica
基金
河南省博士后科研资助项目(2013047)
关键词
乙型脑炎病毒
E蛋白
生物信息学分析
Japanese encephalitis virus
E protein
Bioinformatics analysis
