摘要
目的检测B细胞易位基因(B-cell translocation gene 1,BTG1)在BGC823胃癌细胞中的过表达对其周期、凋亡及迁移的影响。方法采用脂质体法将pc DNA3.1-BTG1重组质粒转染细胞,经过筛选得到稳定表达细胞株后,分为含BTG1质粒组、空载体组和空白对照组,采用Western-blot检测BTG1蛋白在BGC823细胞株中的表达,采用流式细胞术及划痕实验观察BTG1对BGC823细胞周期、凋亡及迁移的影响。结果与空载体组、空白对照组相比,BTG1组蛋白表达增高(P<0.05);流式细胞术显示,BTG1组G2/M期细胞数明显高于其他组(21.38%、18.47%vs 8.57%、8.74%,P<0.05),同时早期凋亡和总凋亡率显著增高(3.41%、3.05%vs 1.32%、0.87%和6.12%、8.30%vs 3.17%、2.34%,P<0.05)。结论 BTG1高表达,诱导G2/M阻滞,抑制细胞增殖,提高凋亡率。
Objective To detect the overexpression of B-cell translocation gene 1（BTG1） in BGC823 and its effect on cell cycle, apoptosis and migration. Methods Recombinant plasmid was transfected into pc DNA3.1-BTG1 cells by Liposome, then the cells were selected and divided into BTG1 plasmid group, empty vector group and blank control group. The expression of BTG1 protein in BGC823 cells was detected by western-blot. The effect of BTG1 overexpression in BGC823 on cell cycle, apoptosis and migration was detected by flow cytometry and wound healing. Results The expression of BTG1 protein was significantly higher in BTG1 group than empty vector group and blank control group（P〈0.05）. Flow cytometry showed that G2 / M phase cells in BTG1 group was significantly higher than other groups（21.38%, 18.47% vs 8.57%, 8.74%, P〈0.05）, while early apoptosis and total apoptosis rate increased significantly（3.41%, 3.05% vs 1.32%, 0.87% and 6.12%, 8.30% vs 3.17%, 2.34%, P〈0.05）. Conclusion High expression of BTG1 can induce the retardation of G2/M, inhibit cell proliferation and increase the rate of apoptosis.
出处
《解放军医学院学报》
CAS
2016年第3期266-270,共5页
Academic Journal of Chinese PLA Medical School
基金
辽宁省科技厅基金(2013020126-215)
辽宁医学院校长基金-奥鸿博泽研究生科研创新基金~~
