摘要
目的观察异丙酚预处理对HK-2细胞缺氧/复氧损伤后水通道蛋白(AQP-1)表达的影响。方法将培养的HK-2细胞随机分为4组:对照组(A组):细胞未经任何处理;氯化钴(CoCl2)组(B组):培养孔中加入300μmol/L的CoCl2处理4 h,然后更换正常的培养基培养24 h,之后更换无血清的培养基培养;脂肪乳剂组(C组):培养孔中加入10%脂肪乳剂90μl预处理1 h,余同B组;异丙酚组(D组):培养孔中加入用DMEM稀释至终浓度25μmol/L的异丙酚预处理1 h,余同B组。MTT法测定细胞增殖,RT-PCR技术检测AQP-1和bcl-2 mRNA的表达。结果经过25μmol/L的异丙酚预处理1 h后,HK-2细胞的增殖明显增高(P<0.01),AQP-1 mRNA的表达明显减少(P<0.01),bcl-2 mRNA的表达明显增加(P<0.01)。结论异丙酚预处理通过调节AQP-1和bcl-2的表达从而减轻HK-2细胞缺氧/复氧后的损伤。
Objective To determine what effects propofol exerts on aqp-1 mRNA expression after HK-2 cells injured by anoxiareoxygenation.Methods HK-2 cells were randomly assigned to one of 4 groups：Control group（group A）,CoCl2 group（group B）.300 μmol/L CoCl2 were added to the cultured HK-2 cells,and then maintained with no serum media for 24 h.In intralipid group（group C）,HK-2 cells were pretreated with 10% intralipid 90 μl 1h and treated as group B.In propofol group（group D）HK-2 cells were pretreated with 25 μmol/L propofol for 1 hour and treated as group B. MTT method was employed to detect the proliferation of HK-2 cells and RT-PCR to show AQP-I mRNA and bcl-2 mRNA expression. Results After pretreated with 25 μmol/L propofol, the proliferation of HK-2 cells was increased （P 〈 0.01 ）. The expression of AQP-1 gene was significantly down-regulated and bcl-2 gene significantly up-regulated（P 〈 0.01 ）. Conclusion Pretreatment with with 25 μmol/L propofol can protect HK-2 cells against anoxia-reoxygenation injury by regulating the expression of AQP-1 mRNA and bcl-2 mRNA.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第8期572-574,共3页
Journal of China Medical University
基金
辽宁省教育厅基金资助项目(20060942)
