摘要
【目的】探讨3,3-二吲哚基甲烷(3,3-diindolylmethane,DIM)对荷鼻咽癌裸鼠肿瘤体积、丙二醛(Malondiadehycle,MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力及凋亡相关蛋白-半胱天冬酶-3信号分子的影响。【方法】采用完全随机的方式将48只BALB/C裸小鼠分为DIM低、中、高(0.004g/kg.b.w、0.02g/kg.b.w、0.1g/kg.b.w)剂量组和溶剂对照组(体积分数为0.5%的羧甲基纤维素钠溶液),分别经口灌喂给予实验动物相应剂量的DIM或对照溶液10d后,于其背部靠腋窝皮下接种人鼻咽癌细胞株CNE1,继续经口灌喂给予实验动物相应剂量的DIM或对照溶液。实验第30天时处死动物,计量肿瘤体积、留取肿瘤组织进行MDA含量、SOD和GSH-Px活力测定、活化型半胱天冬酶-3活性检测。【结果】DIM各剂量可不同程度地抑制肿瘤生长。与对照组比较,高剂量组SOD活力明显升高(P<0.05)、MDA含量明显降低(P<0.05)、GSH-Px活力明显升高(P<0.05)。细胞凋亡的关键执行者活化型半胱天冬酶-3(CleavedCaspase-3)表达随染毒剂量的增加逐渐增强(P<0.05)。【结论】3,3-二吲哚基甲烷可抑制肿瘤生长,其机理可能与通过降低MDA含量,提高SOD活力、GSH-Px活力等途径降低氧化应激程度及通过Caspase-3信号分子诱导凋亡的作用相关。
[Objective ] To observe the effects of 3,3-diindolylmethane （DIM） on the tumor volume, tumor mass, the level of MDA, the activity of SOD and GSH-Px in null mice grafted with nasopharyngeal carcinoma. [Methods] Forty-eight BALB/C null mice were divided into control group （0.5% SCMC）, low dose （0.004 g/kg.b.w）, middle dose （0.02 g/ kg.b.w） and high dose （0.1 g/kg.b.w） of DIM. The mice were administered with different solutions by gavage for 10 days before the tumor were grafted, then the solutions were continue to administered by garage. The tumor volume, tumor mass, the level of MDA , the activity of SOD, the activity of GSH-Px, the level of cleaved Caspase-3 were determined on the 30th day of the research. [Results] Compared with the control group, the content of MDA in the tumor tissue of high dosage group could obviously be reduced and the activity of SOD as well as the GSH-Px activity could be remarkably enhanced （P〈 0.05）. The level of cleaved Caspase-3 seems to be elevated according to the increase of DIM doses. [Conclusion] DIM could reduce the growth of tumor, the mechanism underlie it could be related with the decrease of the content of MDA as well as the elevated level of SOD, GSH-Px, and perhaps could be related with the apoptosis transducted by cleaved Caspase-3.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2009年第A04期60-63,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广州市医药卫生科技项目(2007-YB-118)
广东省医学科学研究基金(20071120440105196106222434)
