摘要
目的建立鸡骨草肝炎颗粒的定性、定量方法。方法采用薄层色谱法定性鉴别处方中的鸡骨草,采用HPLC法测定制剂中的绿原酸和槲皮素。结果鸡骨草薄层鉴别方法分离度好、专属性强、简单可行;绿原酸和槲皮素的线性范围分别为0.0046～0.0276μg.μl^-1（r=0.9999）、0.0015～0.0084μg.μl^-1（r=0.9991）,平均加样回收率分别为98.02%、97.91%,RSD=1.8%、1.1%。结论所建方法可控制鸡骨草肝炎颗粒的质量。
OBJECTIVE To establish a qualitative and quantitative analysis system of Jigucao Hepatitis granules for better quality control.METHODS The TLC technique was used to identify the Herba Abri.HPLC was used to measure the content of the chlorogenic acid and quercetin.RESULTS The TLC identification for Herba Abri was highly specific and simple.There was a good linear relation between the concentration and the peak-areas of chlorogenic acid and quercetin.The linear regression for chlorogenic acid was over the range of 0.0046 to 0.0276 μg·μl^-1（r=0.9999） and the quercetin was over the range of 0.00145 to 0.0084 μg·μl^-1（r=0.9991）.The average recovery rate were 97.88%（RSD=1.7%） and 97.91%（RSD=1.1%）.CONCLUSION The TLC method was feasible and rational.This HPLC method was accurate,reproducible and simple.It could be used for the quality control of Jigucao Hepatitis granules.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2009年第4期409-411,共3页
West China Journal of Pharmaceutical Sciences
基金
广西壮族自治区教育厅立项项目(200710LX006)
