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非创伤性股骨头坏死股骨头局部bFGF与BMP-2 mRNA表达的研究 被引量:7

Expression of bFGF and BMP-2mRNA of the local femoral head in nontraumatic osteonecrosis of femoral head
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摘要 [目的]探讨非创伤性股骨头坏死(nontraumatic osteonecrosis of femoral head,NONFH)股骨头局部碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)与骨形态发生蛋白-2(bone morphogenic proteins,BMP-2)表达的变化情况及其意义。[方法]收集经全髋关节置换术取下的NONFH的股骨头标本30例作为实验组,新鲜股骨颈骨折标本10例作为对照组。于坏死区、健存区取材,经固定、脱钙后,制成切片,光镜观察其病理变化,并运用原位杂交技术分别对其bFGF与BMP-2的表达情况进行检测。[结果]实验组组织结构紊乱,破碎,骨小梁稀疏、不完整,有大量的空骨陷窝,而对照组骨小梁相对较完整、排列整齐,骨小梁中的骨细胞清晰可见,偶见空骨陷窝。实验组中bFGF与BMP-2的阳性表达强度及面积均明显低于对照组,结果有统计学意义(P<0.05)。[结论]NON-FH股骨头局部bFGF与BMP-2表达的降低,极有可能是导致其自身修复能力不足的重要原因。增加股骨头局部bF-GF与BMP-2的表达可能有助于股骨头缺血坏死的修复。 [ Objective] To explore the change and its significance of the expression of basic fibroblastic growth factor ( bF- GF) and bone morphogenetic protein- 2 (BMP- 2) of the local femoral head in nontraumatic osteoneerosis (NONFH) . [ Methods] Thirty samples of femoral heads of NONFH were collected as the experimental group and 10 fresh samples of femoral heads of femoral neck fracture as control group. The specimens were collected at the time of total articular replacement arthroplasty. The bone tissues from necrosis area and healthy area were made into general sections after immobility and decalcification. Pathological changes were exexamined by optical microscopy and the expression of bFGF and BMP - 2 in femoral head was determined with in - situ hybridization technique. [ Results] The organization structure of experimental gronp was disorganized, cracked, and the bone trabecula was rarefactive and non - intact. There were a great number of empty lacune in bone trabecula. The intensity and area of positive expression of bFGF and BMP -2 in femoral head of the experimental group were obviously lower than that of control group. The result had statistical significance (P 〈0. 05 ) . [ Conclusion] The decreased expression of bFGF and BMP -2 in femoral head of NONFH may be reason for insufficient repair function.
出处 《中国矫形外科杂志》 CAS CSCD 北大核心 2009年第15期1180-1183,共4页 Orthopedic Journal of China
基金 宁夏回族自治区自然科学基金项目(编号:NZ08136)
关键词 非创伤性股骨头坏死 碱性成纤维细胞生长因子 骨形态发生蛋白-2 表达 nontraumatic osteonecrosis of femoral head basic fibroblastic growth factor bone morphogenetic protein - 2 expression
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