摘要
目的坏死骨的血管再生及骨修复能力与VEGF、bFGF与BMP-2多种生长因子关系密切,通过观察股骨颈骨折、创伤性及非创伤性股骨头缺血性坏死(avascular necrosis of femoral head,ANFH)的股骨头局部上述因子的表达变化,并与其组织病理学的骨质含量指标进行相关性分析,为进一步探讨ANFH的发病机制及临床针对不同病因进行个性化治疗提供实验依据。方法取59例人工全髋关节置换术患者自愿捐赠的股骨头标本进行观察。创伤性ANFH 22例(A组),Ficat分期:Ⅲ期13例,Ⅳ期9例。非创伤性ANFH 19例(B组),Ficat分期:Ⅲ期11例,Ⅳ期8例;其中激素性10例,酒精性7例,原因不明2例。新鲜股骨颈骨折18例(C组)。3组患者性别、年龄等一般资料比较,差异均无统计学意义(P>0.05)。采用双能X线骨密度仪测量股骨头负重区骨密度;大体观察组织病理学改变,HE染色光镜及扫描电镜下观察其病理变化,计算空骨陷窝百分比及骨小梁面积百分比,并采用原位杂交技术分别对其VEGF、bFGF、BMP-2 mRNA表达进行检测。结果 A、B组骨密度均低于C组,B组低于A组,组间差异均有统计学意义(P<0.05)。A、B组股骨头形态不规则,光镜下见坏死区骨小梁稀疏、不完整,有大量空骨陷窝;健存区A组有较多纤维组织增生,B组髓腔内脂肪细胞增生、肥大。扫描电镜示A、B组大多骨细胞脂肪变性、坏死,骨基质内见脂肪细胞增生。C组均呈正常股骨头结构。A、B组空骨陷窝百分比均高于C组,骨小梁面积百分比均低于C组,差异均有统计学意义(P<0.05);A、B组间仅空骨陷窝百分比差异有统计学意义(P<0.05)。A组与B组VEGF、BMP-2、bFGF mRNA阳性染色面积百分比及吸光度(A)值均明显低于C组(P<0.05);A组BMP-2、bFGF mRNA两指标均较B组高(P<0.05),但VEGF mRNA A、B组间差异无统计学意义(P>0.05)。上述各因子表达强度与骨密度、骨小梁面积百分比成正相关,而与空骨陷窝百分比
Objective To study the expression changes of vascular endothelial growth factor （VEGF）, basic fibroblast growth factor （bFGF）, and bone morphogenetic protein 2 （BMP-2） in femoral neck fracture, traumatic, and non-traumatic avascular necrosis of femoral head （ANFH）, and to study the relationship between the expressions of VEGF, bFGF, BMP-2 mRNA and bone mass so as to explore the pathogenesis of ANFH and provide the exprimental basis for individual treatment of ANFH. Methods Femoral head specimens were obtained from 59 donors undergoing total hip replacement, including 22 cases of traumatic ANFH （group A, 13 cases of Ficat stage III and 9 cases of Ficat stage Ⅳ）, 19 cases of non-traumatic ANFH （group B, 11 cases of Ficat stage Ⅲ and 8 cases of Ficat stage Ⅳ; 10 cases of steroid-induced ANFH, 7 cases of alcoholic ANFH, and 2 cases of unexplained ANFH）, and 18 cases of fresh femoral neck fracture （group C）. There was no significant difference in the general data among 3 groups （P 〉 0.05）. The bone mineral density （BMD） at weight-bearing area of the femoral head was measured with dual energy X-ray absorptiometry. The pathological changes were observed by using optical microscope and scanning electron microscope. The percentage of empty bone lacuna and the percentage of trabecular bone area were calculated. The expressions of VEGF, bFGF, and BMP-2 mRNA in femoral head were detected by use of in-situ hybridization technique. Results The BMD in groups A and B were significantly lower than that in group C （P 〈 0.05）, and there was significant difference between group A and group B （P 〈 0.05）. In the necrosis area of groups A and B, the bone trabecula was rarefactive and not of integrity, with a great number of empty bone lacuna. In healthy area, more fiber hyperplasia was observed in group A, the proliferated and hypertrophic fat cells in the medullary cavity in group B. Scanning electron microscope showed that many osteocytes underwent fatty degeneration and necrosi
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2011年第8期984-991,共8页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
股骨头缺血性坏死
VEGF
BFGF
BMP-2
骨质含量
Avascular necrosis of femoral head Vascular endothelial growth factor Basic fibroblast growth factor Bone morphogenetic protein 2 Bone mass
