摘要
[目的]研究大剂量甲强龙(糖皮质激素)对股骨头、股骨近端组织BMP-2表达的影响,探索ONFH行人工关节置换治疗翻修率较高的原因。[方法]健康新西兰成年大白兔45只,随机分为1个对照组(A组),2个实验组(B、C组),每组均15只,B组连续3 d,C组连续6 d肌注甲强龙80 mg/(kg.d),诱发股骨头坏死模型,分别于注射后2、4、8周切取双侧股骨头、小转子处约3 mm厚的股骨近端段,行SABC法免疫组化染色,观察BMP-2表达情况。[结果]对于股骨头、股骨近端骨,激素使用剂量或作用时间,均可致BMP-2的表达减少,且激素使用剂量与作用时间有交互作用,(P<0.05)。[结论]大剂量甲强龙可以造成股骨头、股骨近端骨组织内BMP-2表达降低,激素的应用时间越长,应用总量越大,对其骨骼的影响越严重。
[ Objective ] To study the effects of high dosage of methylprednisolone on acetabulum, caput ossis femoris and proximal femurs in a rabbit model by expression of BMP - 2. [ Method ] Twenty-seven adult rabbits were divided into three groups. Besides control group （ group A）, experimental groups were consisted group B and group C, with 9 rabbits in each group. The animal model of osteonecrosis of the femoral head（ONFH） was established by intramuscular injection of methylpred- nisolone sodium succinate 80 mg/kg/d in rabbits, for 3 days in group B, for 6 days in group C. At 2,4,8 weeks after steroid administration, the bone tissue of caput ossis femoris and proximal femurs were collected and the changes of expression of BMP -2 were observed. [ Result]The average percentage of dyed areas and average optical density of BMP- 2 had a trend of de- creasing with time and dosages increased in group B, C. No dyed areas was observed in individual samples （ P 〈 0.05 ）. [ Conclusion] High dosages of methylprednisolone can lead to osteonecrosis of femoral head and proximal femurs, reduce the expres- sion of BMP - 2. The higher the dosages of methylprednisolone are administered and the longer the administering time lasts, the poorer of the bone of hip and the proximal femur will be.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2012年第16期1502-1504,共3页
Orthopedic Journal of China
