摘要
以小尾寒羊(48只)、宁夏滩羊(121只)、滩寒杂交羊F1(23只)、无角陶塞特(48只)、萨福克(24只)5个绵羊群体为实验材料,利用PCR-SSCP和DNA测序技术对心脏型脂肪酸结合蛋白(H-FABP)基因(GenBank登录号:AY157617)外显子2和内含子2部分序列进行单核苷酸多态性(SNPs)检测及遗传多态性分析。结果表明:(1)引物2的PCR扩增产物中存在981(G/A)、1014(A/C)、1019(T/C)和1058(-/G)4个SNP位点,表现为AA、BB、CC、AB、AC、BC、AD、CD和BD9种基因型,其中AA为优势基因型。经χ2检验后,除滩羊和萨福克羊外,其他群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态。群体遗传多态性分析表明:宁夏滩羊、小尾寒羊和无角陶塞特羊3个群体中的多态信息含量(PIC)均处于0.25和0.50之间,为中度多态,萨福克羊和滩寒杂交羊F1为低度多态(PIC<0.25),表明脂肪酸结合蛋白基因在不同绵羊品种中具有单核苷酸多态性,可以进一步作为候选基因来分析其与肌内脂肪含量性状的关联性。(2)引物4的PCR扩增产物中检测到1个SNP多态位点为2407(T/C),表现为HH、Hh和hh3种基因型,基因型频率大小为HH>Hh>hh,经χ2检验后,在滩羊和无角陶塞特羊中均为达到Hardy-Weinberg平衡状态,其多态信息含量均为低度多态(PIC<0.25),而在小尾寒羊、滩寒杂交羊F1和萨福克羊均没有多态出现。
PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms （SNPs） and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP（Heart fatty acid-binding protein）gene, and five sheep populations that comprised of Small-Tailed Han sheep （SH, 48）, Ningxia Tan sheep （Tan, 121）, Tan x SH F1 （23）, Poll Dorset （48） and Suffolk （24） sheep were screened in this stuty. The result showed： （1）four SNPs at 981（G/A）, 1014（A/C） 1019（T/C） and 1058 （-/G） and nine genotypes （AA, BB, CC, AB, AC, BC, AD, CD and BD） were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content （P1C） in the Suffolk and Tan x SH F1 populations （PIC〈 0.25） but an intermediate PIC in the remaining three populations （0.25〈 PIC 〈0.50）. It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. （2）Three genotypes （HH, Hh and hh） determined by a SNP at 2407（T-C） were de- tected using primer 4. The genotype frequencies were in the order of HH 〉 Hh 〉 hh. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low （PIC 〈 0.25）. However, there was no polymorphisms in SH, Tan x SH Fl and Suffolk populations.
出处
《遗传》
CAS
CSCD
北大核心
2009年第7期725-731,共7页
Hereditas（Beijing)
基金
“十一五”国家科技支撑项目(编号:2006BAD13B08)
国家高技术研究发展计划(863计划)项目(编号:2006AA10Z198)资助
