摘要
目的研究宁夏回、汉族亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与冠心病之间的关系。方法对回、汉族186例冠心病患者和175例对照者,应用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)分析检测MTHFR基因C677T多态性。结果回、汉族冠心病组和对照组MTHFR C677TCC、CT、TT三种基因型分布(回族,χ2=5.523,P=0.063;汉族,χ2=3.810,P=0.149)和等位基因频率分布(回族,χ2=0.000,P=0.984;汉族,χ2=1.366,P=0.243)差异无统计学意义;冠心病组和对照组内回、汉不同民族基因型分布和等位基因频率分布差异亦无统计学意义。结论亚甲基四氢叶酸还原酶基因MTHFR C677T存在多态性,回、汉族间差异无统计学意义。
Objective To study the correlation between gene polymorphism of methylenetetrahydrofolate reductase （MTHFR） C677T gene and Coronary Heart Disease （CHD） in Ningxia Hui and Han ethnic groups. Methods In 186 CHD patients and 175 control subjects from Hui and Han ethnic groups, polymerise chainreaction- restriction fragment length polymorphism （PCR -RFLP） was applied to analyze the polymorphism of MTHFR gene C677Results There was no statistical significance in the genotype distribution （Hui ethnic group χ^2 = 5. 523 ,P = 0. 063; Han ethnic group χ^2 = 3. 810, P = 0. 149 ） and allele frequency distribution （Hui ethnic group χ^2 = 0. 000, P = 0. 984; Han ethnic group χ^2 = 1. 366, P = 0. 243 ） of MTHFR C677T between CHD group and the control group in Hui and Han ethnic groups and there was no statistical significance in the genotype distribution and allele frequency distribution between the two ethnic groups within GHD group and the control group. Conclusion There is polymorphism in gene MTHFR C677T. There is some difference between the two ethnic groups.
出处
《宁夏医学杂志》
CAS
2009年第6期494-496,F0002,共4页
Ningxia Medical Journal
关键词
回族
汉族
亚甲基四氢叶酸还原酶
冠心病
基因多态性
Hui ethnic groups
Han ethnic groups
Methylenetetrahydrofolate reductase
Coronary Heart Disease
Gene polymorphism
