摘要
目的研究N5,N10亚甲基四氢叶酸还原酶基因多态性及其与冠心病的关系。方法运用多聚酶链反应限制片段长度多态性技术检测143例广东汉族正常人和47例冠心病患者N5,N10亚甲基四氢叶酸还原酶基因C677T多态性。结果N5,N10亚甲基四氢叶酸还原酶有三种基因型,即纯合子突变型(TT)、杂合子突变型(TC)和正常型(CC)。正常组TT型频率为3%,TC型频率为36.5%,CC型频率为60.5%,T等位基因频率为19.6%,C等位基因频率为80.4%;冠心病组TT型频率为4.2%,TC型频率为23.4%,CC型频率为72.4%,T等位基因频率为13.9%,C等位基因频率为86.1%。两组等位基因频率经χ2检验差异无显著意义(P>0.05)。结论C677T可能不是广东汉族人冠心病的危险因素,但结果仍有待研究。
Objective To study the relationship between polymorphisms of methylenetetrahydrofolate(MTHFR) gene and coronary heart disease(CHD).Methods MTHFR C677T genotypes of 47 CHD patients and 143 normal subjects were analyzed by polymerase chain reaction and restriction fragment length polymorphism.Results There were three kinds of genotypes:TT(homozygous mutation),TC(heterozygous mutation),CC(wild type).The frequencies of the three kinds of genotypes were as follows:TT,3%;TC,36.5%;CC,60.5% in normal subjects and TT,4.3%;TC,23.4%;CC,70.3% in CHD patients.The frequencies of the allele T in the two groups were 19.6% and 13.9% respectively.No significant difference was found between the two groups in χ2-test(P>0.05).Conclusion MTHFR C677T gene mutation may not be a risk factor for coronary heart disease in Han nationality Guangdong province,but a further study is still needed.
出处
《中国基层医药》
CAS
2005年第6期661-662,共2页
Chinese Journal of Primary Medicine and Pharmacy
