摘要
目的探讨白细胞介素6(interleukin6,IL-6)基因多态性在宁夏回、汉族人群中的分布特征。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对210例回族和308例汉族人群白细胞介素6基因启动子区三个多态性位点(-572G/C、-597G/A、-174G/C)进行检测。结果宁夏回族群体IL-6基因三个SNP各基因型频率及等位基因频率分别为,-572G/C(CC:55.71%;CG:34.76%;GG:9.52%;C:73.10%;G:26.90%);-597G/A(GG:94.29%;GA:5.71%;AA:0%;G:97.14%;A:2.86);-174G/C(GG:96.67%;GC:3.23%;CC:0%;G:98.33%;C:1.67%)。宁夏汉族群体IL-6基因三个SNP各基因型频率及等位基因频率分别为,-572(CC:52.92%;CG:35.71%;GG:11.36%;C:70.78%;G:29.22%);-597(GG:92.86%;GA:7.14%;AA:0%;G:96.13%;A:3.57);-174(GG:97.08%;GC:2.92%;CC:0%;G:98.54%;C:1.46%)。两组间差异均无统计学意义(P>0.05);按性别分组,三个SNP位点的基因型频率及等位基因频率差异亦无统计学意义(P>0.05)。结论 IL-6基因-572、-597、-174三个多态性位点在宁夏回、汉族人群及不同性别间差异无统计学意义。
Objective To explore the frequencies of allele and genotype distribution of interleukin6 （ IL -6） gene promoter-572G/C, -597G/A and - 174G/C polymorphisms in the Hui and Han people of Ningxia Province and to analyze the distributions of IL -6 polymorphism among different places and ethnic groups. Methods The gene IL-6 -572G/C, -597G/A and -174G/C polymorphisms was examined by the polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP） methods in 210 hui and 308 han healthy adults. Results IL -6 -572, -597, -174 genotype frequencies were -572 （ CC ：55.71% ; CG：34.76% ;GG：9.52% ;C：73. 10% ;G：26.90%）, -597 （GG：94.29%;GA：5.71% ;AA：0% ;G： 97. 14 % ; A ：2.86 ） and - 174 （ GG： 96.67 % ; GC ： 3.23 % ; CC ： 0 % ; G： 98.33 % ; C ： 1.67 % ） in Ningxia Hui ethnic group, respectively. IL -6 -572, - 597, -174 genotypes frequencies were -572 （ CC ：52.92% ; CG ： 35.71% ;GG：11.36% ;C：70.78% ;G：29.22%）, -597（GG：92.86% ;GA：7.14% ;AA：0% ;G：96.13% ; A：3.57）, -174（GG：97.08%;GC：2.92%;CC：0%;G：98.54%;C：l.46%） in Ningxia Han ethnic group, respectively. The Phenotype frequencies and allele gene frequencies of IL -6 gene showed no significant difference between the Hui and Han ethnicity in Ningxia （P 〉 0.05 ）. The Phenotype frequency and allele gene frequency of IL -6 gene showed significant difference between the females and males in Ningxia. （P 〉 0.05 ）. Conclusion The three SNPs of IL - 6 genes distribution among the Hui and Han ethnicity of Ningxia have no significant difference.
出处
《宁夏医科大学学报》
2011年第5期449-454,共6页
Journal of Ningxia Medical University
基金
宁夏自然科学基金(NZ0990)
宁夏科技公关项目(2007GG071)
关键词
白细胞介素6
基因多态性
回族
汉族
interleukin 6
gene polymorphism
Hui Ethnic
Han Ethnic
