摘要
目的 从病因学角度探讨白细胞介素-6(IL-6)基因启动子-572C/G多态性与2型糖尿病并发下肢血管病变间的关系.方法 选取2008年2月至2009年1月天津医科大学总医院内分泌科住院的2型糖尿病患者280例,其中男130例,年龄(58.7±0.8)岁;女150例,年龄(58.1±0.5)岁.根据血管彩色多普勒超声检查分为下肢血管病变(VDLE)组、下肢血管神经病变(VNDLE)组、下肢神经病变(NDLE)组和无下肢并发症(NC)组.另选取2008年12月至2009年1月社区健康查体正常的人群70例作为正常对照组.取受试者静脉血提取基因组DNA,聚会酶链反应-限制性片段长度多态性(PCR-RFLP)法测定IL-6启动子区-572C/G基因多态性.各组间频率比较用X2检验,不同组间的计量资料均数比较采用单因素方差分析.结果 IL-6启动子-572C/G具有多态性分布的特点.基因型GG和CG,等位基因G频率在糖尿病组(32.1%CC,49.3%CG,18.6%GG;43.2%G,56.8%C)高于正常对照组(54.3%CC,37.1%CG,8.6%GG;27.1%G,72.9%C),差异有统计学意义(X2值分别为12.649和12.054,P<0.05);在糖尿病各组和正常对照组间差异有统计学意义(X2值分别为22.015和20.471,P<0.05);在下肢血管病变组(23.4%CC,53.9%CG,22.7%GG;49.6%G,50.4%C)高于无下肢血管病变组(36.7%CC,47.5%CG,15.8%GG;60.4%G,39.6%C),差异有统计学意义(X2值分别为6.399和5.752,P<0.05).结论 IL-6启动子-572C/G的基因型GG和CG,等位基因G可能是2型糖尿病合并下肢血管病变的易感基因,IL-6启动子-572C/G基因多态性与2型糖尿病下肢血管病变密切相关.
Objective To investigate the polymorphism of the interleukin-6 promoter-572C/G and its relationship with type 2 diabetes mellitus(T2DM)complicated with vascular disease of lower extremities.Methods Two hundreds and eighty hospitalized T2DM patients from the Department of Endocrinology in General Hospital of Tianjin Medical University were recruited from February 2008 to January 2009.One hundred and thirty cases were male in(58.7±0.8)years and 150 cases were female(mesh age(58.1 ±0.5)years).Based upon the ultrasound test of the lower extremities.the participants were divided into vascular disease of lower extremities(VDLE)group,vascular and neuropathic disease of lower extremities (VNDLE)group,neuropathic disease of lower extremities(NDLE)group and non-complications group (NC).Seventy people for health care were non-randomly recruited as healthy control group.By PCR-RFLP technique.the polymorphism of the human IL-6 gene-572C/G in 70 health controls and 280 type 2 diabetic patients was evaluated.The genotype and allele frequency in different groups was evaluated by X2 teal The measurement data in different groups was evaluated by analysis of variance.Results The IL-6 promoter572C/G had polymorphism.The frequeney of genotype GG and CG,as well as allele G,had statistics difference between T2DM group and healthy control group(X2=12.649,12.054,respectively,all P<0.05).And so did they in T2DM patients who complicated with different vascular disease of the lower extremities compared with healthy control group(X2=22.015,20.471,respectively,all P<0.05).The genotype and allele frequency was also higher in T2DM patients complicated with vascular disease of lower extremities than those without the complications(X2=6.399,5.752,respectively,all P<0.05).Conclusion The genotype GG and CG and the allele G of the Interleukin-6 promoter-572C/G might be the susceptibility gene of the T2DM complicated with vascular disease of the lower extremities.The polymorphism of the Interleukin-6 promoter-572c/G might has great correlation with
出处
《中华糖尿病杂志》
CAS
2010年第3期-,共4页
CHINESE JOURNAL OF DIABETES MELLITUS
