摘要
目的评估湖北和河南两地区汉族人群Nei核酸内切酶VIII样蛋白3(NEIL3)基因rs12645561多态性位点与冠状动脉硬化严重程度的相关性。方法以947例经冠状动脉造影病例为研究对象,采用病变支数得分和Gensini得分评估冠状动脉硬化严重程度;采用高分辨率熔解曲线进行rs12645561基因分型;ELISA法检测血浆NEIL3蛋白水平。结果 rs12645561 T风险等位可增加冠心病风险(χ2=12.165,P<0.05),并与病变支数得分和Gensini得分明显相关(χ2分别为14.745和15.615,P<0.05);rs12645561 CT+TT风险基因型、体重指数>25 kg/m2、高脂血症和吸烟是Gensini得分增高的独立危险因素(OR分别为1.50、1.54、2.01、1.42,P<0.05);血浆NEIL3蛋白表达水平与rs12645561基因型分布、病变支数得分和Gensini得分呈负相关(P<0.05)。结论 rs12645561与湖北和河南地区冠状动脉硬化严重程度密切相关,并可影响NEIL3蛋白表达。
Objective To assess the association of polymorphism at rs12645561 locus of Nei endonuclease Ⅷ-like 3（ NEIL3） gene with the severity of coronary atherosclerosis in Han population of Hubei and Henan regions in central China. Methods In 947 cases undergoing coronary angiography,the severity of coronary atherosclerosis was calculated by lesion vessel number scores and Gensini scores.The genotypes of rs12645561 were analyzed by high resolution melting curve. The plasma NEIL3 levels were measured by enzyme-linked immunosorbent assay. Results The minor allele T of rs12645561 was significantly associated with the risk of coronary artery disease（ χ^2= 12.165,P〈0.05） including increased lesion vessel scores and Gensini scores（ χ^2= 14. 745 and 15. 615,P〈0. 05）.The variant risk genotypes（ CC＋CT） of rs12645561,body mass index of more than 25 kg/m2,hypelipidemia and smoking habit were all the independent risk factors for higher Gensini scores（ OR = 1.50,1.54,2.01 and 1.42,respectively,P〈0.05）. There were significantly inverse correlations of plasma NEIL3 levels with the distribution of rs12645561,lesion vessel number scores and Gensini scores（ P〈0.05）. Conclusion rs12645561 may correlate with the severity of coronary atherosclerosis,and contribute to the development of coronary atherosclerosis of Han population of Henan and Hubei regions. rs12645561 may also affect the levels of NEIL3 protein.
作者
崔宁华
王雪玢
张帅
李聪
CUI Ninghua;WANG Xuebin;ZHANG Shuai;LI Cong(Department of Clinical Laboratory, Children＇s Hospital Affiliated to Zhengzhou University, Henan Children＇s Hospital, Zhengzhou Children＇s Hospital, Zhengzhou 450053 ,Henan;Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan;Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China)
出处
《临床检验杂志》
CAS
CSCD
2018年第5期332-336,共5页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金(81300154)
