摘要
目的:探讨糖蛋白A34在胃癌演变过程中的表达情况及临床意义。方法:采用RT-PCR方法分别检测18例正常胃黏膜(A组)、30例慢性萎缩性胃炎胃黏膜(B组)、24例早期胃癌黏膜组织(C组)及48例进展期胃癌黏膜组织(D组)中糖蛋白A34 mRNA的表达情况。免疫组化法检测各组糖蛋白A34的表达情况。结果:糖蛋白A34 mR-NA在正常组织、萎缩性胃炎组织、早期胃癌组织和进展期胃癌组织中相对表达水平分别为0.093±0.010,0.211±0.023,0.258±0.017,0.259±0.021,糖蛋白A34在各组表达阳性率分别为5.56%,33.33%,70.83%,75.00%。糖蛋白A34及其mRNA的表达在A,B,C三组之间差异均具有统计学意义(P<0.05),早期胃癌和进展期胃癌两组之间差异无统计学意义(P>0.05)。结论:糖蛋白A34在胃癌的演变过程中的表达逐渐加强。
Objective： To investigate the expression of glycoprotein A34 in the development of gastric carcinoma and discuss their relations with clinical significance. Methods： The expression of glycoprotein A34 mRNA and protein was detected by RT-PCR and immunohistochemical technique in 18 healthy volun- teers （group A） ,30 patients with chronic atrophic gastritis（ group B）, 24 patients with early gastric carcinoma（ group C）, and 48 patients with advanced gastric carcinoma（ group D）. Results： The expression of gly coprotein A34 mRNA were0. 093 ± 0. 010, 0.211 ±0. 023, 0. 258 ± 0. 017 and O. 259 ＋ 0.021, in normal, chronic atrophic gastritis, early gastric carcinoma and advanced gastric carcinoma respectively. The positive rate of glycoprotein A34 were 5.56% , 33.33% , 70.83% , 75.00% , respectively. There were statistical difference among group A, B and C （P 〈 0.05 ） and no statistical difference between group C and D （ P 〉0. 05 ）. Conclusion： The expression of glycoprotein A34 were enhanced gradually during the course of early gastric cancer being developed.
出处
《江苏大学学报（医学版）》
CAS
2009年第3期240-242,共3页
Journal of Jiangsu University：Medicine Edition
基金
镇江市社会发展计划资助项目(SH2006034)
