摘要
目的研究p53、bcl2、cerbB2基因在胃癌及癌前病变中的表达,探讨其与胃癌发生的关系。方法采用免疫组化SABC法,检测p53、bcl2、cerbB2基因蛋白在98例胃癌、39例肠上皮化生、40例不典型增生和20例正常胃粘膜中的表达。结果①p53在胃癌中的阳性表达率为55.1%,早期胃癌和进展期胃癌分别为50.0%,55.4%;在肠上皮化生中的表达率为12.8%,与早期胃癌比较有统计学差异(P<0.05),随不典型增生程度的加重p53的表达率逐渐升高,依次为5%,20%,60%,其中轻中度与重度不典型增生差异有统计学意义(P<0.05),而在正常胃粘膜中未见p53表达②bcl2在正常胃粘膜中有弱阳性表达(10.0%),在不典型增生中为50.0%,在胃癌中为44.9%,不典型增生与胃癌中的表达均显著高于正常胃粘膜(P<0.05)。③cerbB2在胃癌中的阳性表达率为55.1%,其中在早期胃癌中的表达率较低(16.7%),在重度不典型增生和进展期胃癌中的表达率较高,分别为60.0%和57.6%,二者与早期胃癌比较差异有统计学意义(P<0.05)。④cerbB2表达与肿瘤浸润深度、淋巴结转移有关(P<0.05),与胃癌分化程度无关(P>0.05);bcl2表达与胃癌的分化程度有关(P<0.05),低分化者bcl2表达率高,与淋巴结转移,浸润深度无关(P>0.05);p53表达与上述临床病理因素均无关(P>0.05)。结论p53,bcl2。
Objective： To explore the expression of p53 ,bcl-2 ,c-erB-2 genes in gastric cancer（GC）and precancerous lesions and define whether the expression of the genes were associated with the stomach carcinogenesis. Methods： immunohistochemical technique SABC method was used to detect the expression of p53 ,bcl-2 ,c-erbB-2 in 98 cases of G0,39 cases of intestinal metaplasia,40 cases of dysplasia and 2o cases of normal gastric mucosa. Results ： 1. The expression of p53 gene was detected in GO （ 55. 196） and the early stage （50.96）, the advanced stage （55. 496） of GO, it also was seen in intestinal metaplasia （12.8%）, there was significant diffenence between the intestnal metaplasia and the early stage of GC（P〈0.05）.The positive rate of p53 expression was 5%,20%,60% in mlid,moderate and severe epitheilal dysplasia（P〈0.05）.respectively.The p53 expression was not found in nermat stornach muccsa 2.The bcl-2 expression was weaky obsevved in 20 control xubjects,but it was strong in gastric epithellat dysplasia（50%）and GC（44.9%）,significant difference was seen between dyspiasia and normal gastric mucosa,betwwn GC and normal glastric muccsa（P〈0.05）.3.The expression of c-erbB-2 gene was found in GC（56.1%）and low in the early stage（16.7%）of GC,put high in ghe acvaced stage（58.6%）and the severe epithelial dyspiasia （60%）,there was a significant difference between the two groups and the early stage of GC（P〈0.05）.4.The expression of c-erbB-2 had something to co with lympb mode metastasis ,depth of invasion（P〈0.05）and anything to do with differentiation（P〉0.05）;no correlation between bcl-2 expression and lyoh node metstais,depth of invasion（P〉0.05）was cbserved.Bcl-2 expression was retated with defferentlation of GC（P〈0.05）.the p53 expression was not corretated with the clinical pathciogical faotors（P〉0.05）.Conclusion：p53,bcl-2,c-erbB-2 may be involved in the develpment of GC.
出处
《中国现代普通外科进展》
CAS
2005年第1期37-39,42,共4页
Chinese Journal of Current Advances in General Surgery
