摘要
目的研究蛋白激酶B(protein kinase B,PKB)小干扰RNA(siRNA)转染人胃癌SGC-7901细胞,探讨转染后抑制癌细胞转移侵袭的可行性.方法应用基因转染技术将蛋白激酶基因片段转染至胃癌SGC-7901细胞中,采用Millicell小室、集落形成实验、流式细胞术等方法观察蛋白激酶BsiRNA转染后对转染组细胞侵袭力的影响.结果与对照组比较转染组的胃癌SGC-7901细胞克隆增殖速度和侵袭能力明显减弱(P<0.01),提示转染组siRNA能特异性抑制蛋白激酶B基因的活化,即蛋白激酶B基因的siRNA片断可抑制胃癌SGC-7901细胞的克隆增殖和癌细胞转移侵袭.结论应用RNAi使蛋白激酶B基因沉默能有效抑制人胃癌SGC-7901细胞的克隆生长,并在一定程度上阻止了癌细胞的侵袭和转移,其作用机理值得进一步的深入研究.
Objective To study the effect of PKB gene （Akt2） silencing on the invasion of gastric cancer cell line SGC-7901 in vitro, and to investigate feasibility of small interfering RNA targeting PKB gene in inhibiting invasion of gastric cancer. Methods The siRNA of PKB gene was transfected into SGC-7901 cell, the ability of SGC - 7901 invasion in vitro was assessed by cell migration and invasion assay in Boyden chamber, clone form rate was observed by clone form assay and the change of cell cycle distribution and apoptosis of SGC-7901 ceils was detected by flow-cytometry. Results After treatment with PKB siRNA, compared with control group, Akt2 siRNA group showed lower invasion ability （ P 〈 0.01） and decreased clone form rate in vitro （ P 〈 0.01） with PKB gene silencing. Conclusions Small interfering RNA targeting PKB gene may become one of effective methods in inhibiting clone form, migration and invasion of gastric cancer. It appears that PKB can be a novel target for the treatment of gastric cancer. It＇ s mechanism of inhibiting invasion ability deserves further study.
出处
《昆明医学院学报》
2009年第5期43-46,共4页
Journal of Kunming Medical College
