摘要
目的:观察沙利度胺对人结肠癌细胞HCT116增殖和迁移的影响,并初步探讨其影响机制。方法:分别用不同浓度沙利度胺处理人结肠癌细胞HCT116,采用MTT法检测沙利度胺对人结肠癌细胞增殖的影响,平板克隆试验法检测沙利度胺对单个细胞克隆增殖的作用,流式细胞术检测细胞周期分布,Transwell细胞培养小室检测沙利度胺对细胞迁移的影响,蛋白免疫印迹检测沙利度胺对细胞P53、CXCR4蛋白的作用。结果:MTT检测显示不同浓度沙利度胺对HCT116细胞增殖无影响,平板克隆试验表明其对细胞克隆生长具有抑制作用,流式细胞检测显示100 mg/L沙利度胺处理组G2/M期细胞比例明显高于对照组(P<0.05),沙利度胺组HCT116细胞迁移数明显少于对照组,P53蛋白、CXCR4蛋白表达与对照组比较无明显变化。结论:沙利度胺抑制结肠癌细胞克隆增殖的作用,可能与其影响细胞周期有关;抑制结肠癌细胞迁移的作用,与CXCR4蛋白表达无关。
Objective：To investigate the effects and mechanisms of thalidomide on human colorectal cancer cell HCT116.Methods：HCT116 cells were treated with different concentration of thalidomide.The influence on proliferation was measured by MTT assay.The effect on single colorectal cell colon was detected with plate colony formation assay.Flow cytometry（FCM） was conducted to determine the effect on cell cycle of HCT116 cells.The changes of migration capability were evaluated using transwell assay.The levels of P53 and CXCR4 proteins were detected by western blotting.Results：The result of MTT assay showed thalidomide had no effect on colon cancer cells.Thalidomide inhibited the plate colony formation of HCT116 cells.FCM showed that cells treated with thalidomide were arrested in G2/M phase.The migration capability was decreased significantly,which was independent of CXCR4 protein expression. Conclusion：The inhibition effect of thalidomide on the colony formation of colorectal cancer cell line is likely to be associated with cell cycle arrest.The migration inhibition effect of thalidomide is independent of CXCR4 protein expression.
出处
《新医学》
2011年第6期374-376,385,F0003,共5页
Journal of New Medicine
关键词
沙利度胺
结肠癌
克隆形成
细胞周期
迁移
Thalidomide
Colorectal cancer
Colony formation
Cell cycle migration
