摘要
目的检测蛋白激酶B(Akt)和15-脂氧合酶-1(15-lipoxygenase-1,15-LOX-1)在胃癌及癌旁正常组织中的表达,以探讨二者在胃癌形成中的临床意义。方法采用RT-PCR法检测新鲜胃癌及癌旁正常组织标本中Akt-1、Akt-2、Akt-3和15-LOX-1 mRNA的表达;Western blot印迹法检测磷酸化Akt(p-Akt)和15-LOX-1蛋白的表达。结果Akt-1、Akt-2、Akt-3 mRNA在胃癌及癌旁正常组织中的表达水平差异均无统计学意义(P>0.05)。p-Akt蛋白在胃癌组织中的表达显著高于癌旁正常组织(P<0.05)。15-LOX-1蛋白及mRNA在胃癌组织的表达均低于癌旁正常组织(P<0.05)。p-Akt蛋白表达与胃癌的肿瘤大小、淋巴结转移和TNM分期呈正相关(P<0.05)。相关性分析表明:胃癌组织中15-LOX-1蛋白表达和p-Akt水平间无明显相关性(r=-0.252,P>0.05)。结论Akt在胃癌的发生发展中具有促进作用。15-LOX-1蛋白可能对胃癌的发生发展有一定抑制作用,但与Akt无直接关系。
Objective To investigate the expressions of PKB/Akt and 15-lipoxygenase-1（15-LOX-1） in gastric cancer and its clinical significance.Methods Reverse transcription polymerase chain reaction（RT-PCR） was used to detect the expressions of Akt-1,Akt-2,Akt-3 and 15-LOX-1 mRNA,and Western blot was conducted to evaluate phosphorylated Akt and 15-LOX-1 protein in fresh gastric carcinoma tissue and normal tissue.Results The expression levels of Akt-1,Akt-2 and Akt-3 mRNA in gastric carcinoma tissue were no significant difference compared with normal tissues（P〉0.05）.The expression of p-Akt protein was significantly higher than that in the normal tissues（P〈0.05）.The expressions of 15-LOX-1 mRNA and protein in gastric carcinoma were significantly lower than those in the normal tissue（P〈0.05）.There was a significant positive correlation between p-Akt protein expression and clinical pathological characters such as tumor size,lymph node metastasis and TNM stage（P〈0.05）.There was no significant correlation between the expressions of 15-LOX-1 and p-Akt protein（r=-0.252,P〉0.05）.Conclusion The 15-LOX-1 protein may restrain the genesis and progress of gastric carcinoma,while the Akt protein may promote the genesis and progress of gastric carcinoma,but there is no direct relation between the 15-LOX-1 protein and Akt protein.
出处
《胃肠病学和肝病学杂志》
CAS
2009年第10期938-941,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
福建省科技人才创新基金资助项目(No.2002J061)
