摘要
【目的】探讨环氧化酶2(COX-2)在膀胱移行细胞癌的表达与微血管、淋巴管生成的关系及其预后意义。【方法】应用免疫组化方法检测81例膀胱移行细胞癌组织中COX-2的表达,同时应用CD34、D2-40分别显色微血管和淋巴管进行微血管和淋巴管计数。并分析其与肿瘤临床病理特征和预后的关系。【结果】COX-2表达阳性率为67.9%。COX-2表达与肿瘤分级呈正相关关系(P=0.016),COX-2表达阳性组MVD和瘤内淋巴管(ITLVD)阳性的比例较阴性组高,两组差异有统计学意义(P=0.046,P=0.030)。瘤内淋巴管阳性与肿瘤分级(P=0.001)和分期(P<0.001)明显相关,瘤旁淋巴管(PTLVD)表达与肿瘤分期(P=0.003)呈正相关。Kaplan-Meier分析表明MVD和瘤内、瘤旁淋巴管阳性对疾病特异性生存有明显的影响(P<0.001,P=0.024和P=0.004),COX-2和瘤内淋巴管阳性者无瘤生存时间较短(P=0.013,P=0.001)。多因素分析表明COX-2表达是膀胱移行细胞癌病人无瘤生存独立的预后因素。【结论】COX-2可能参与膀胱移行细胞癌的发生并促进血管及淋巴管生成,影响肿瘤的复发、进展和预后。
[Objectives] The aim of the study was to investigate the expression of cyclooxygenase-2 （COX-2） and its correlation with lymphangiogenesis and angiogenesis in bladder transitional cell carcinoma （TCC）, as well as to analysis the correlation with clinicopathological parameters and prognosis. [Methods] COX-2 immunostaining was undertaken on serial tissue sections from 81 patients with bladder TCC. Microvessel density （MVD） and lymphatic vessel density （LVD） were determined by counts of microvessels, peritumoral lymphatic vessel ducts （PTLVD） and intratumoral lymphatic vessel ducts （ITLVD） stained by CD34 and D2-40 respectively. Their relationship with clinicopathological findings and patient＇s survival time were also analyzed. [Results] The positive rate of COX-2 was 67.9%. COX-2 immunoreactivity was significantly associated with tumor grade （P = 0.016） and also correlated to MVD （P = 0.046） and the presence of ITLVD （P = 0.030）. The presence of ITLVD was significantly correlated with tumor grade （P = 0.001 ） and stage （P 〈 0.001 ）, and PTLVD correlated with tumor stage （P = 0.003）. Kaplan-Meier analysis showed that MVD and the presence of ITLVD and FFLVD were significantly correlated with disease specific survival （P = 0.004, P 〈 0.001 and P = 0.024, respectively）, and both COX- 2 and the presence of ITLVD indicated poor disease free survival （P = 0.013 and P = 0.001, respectively）. Multivariate analysis using Cox regressive model showed that COX-2 was an independent prognostic factor for disease free survival. [Conclusion] These results suggest that overexpression of COX-2 may contribute to tumorigenesis and may have a function on promoting angiogenesis and lymphangiogenesis in bladder TCC, hence affect tumor recurrence, progression and prognosis.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2009年第3期299-303,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省自然科学基金(5001776)
广州市科技计划项目(2005Z3-E0381)
关键词
膀胱移行细胞癌
环氧化酶2
微血管生成
淋巴管生成
bladder transitional cell carcinoma
cyclooxygenase-2
angiogenesis
lymphangiogenesis
