摘要
目的探讨血管内皮生长因子-C(VEGF-C)、血管内皮生长因子受体-3(VEGFR-3)在结直肠癌组织中的表达及其与临床病理参数之间的关系。方法采用免疫组织化学法,检测63例结直肠癌患者(病例组)及20例良性腺瘤及周围正常结肠组织(对照组)中微淋巴管密度(LMVD)及VEGF-C、VEGFR-3的表达;分析LMVD、VEGF-C和VEGFR-3表达与临床各病理参数之间的关系。结果病例组LMVD高于对照组(14.82±4.41 vs 9.56±2.32.P<0.01),病例组中LMVD在有淋巴结转移、TNM分期较高、VEGF-C和VEGFR-3阳性表达的结直肠癌组织中表达较高,差异有统计学意义(P<0.01),而不同性别、年龄、组织分型和分化程度的患者中差异无统计学意义(P>0.05)。VEGF-C和VEGFR-3在病例组的表达均高于对照组(分别为60.32%vs 35.00%和71.43%vs 40.00%,均为P<0·01),病例组VEGF-C和VEGFR-3的表达在有淋巴结转移和TNM分期较高的患者中明显升高,差异有统计学意义(P<0.01),而在不同性别、年龄、组织分型和分化程度的患者中差异无统计学意义(P>0.05)。结论 VEGFC和VEGFR-3与结直肠癌的发展及转移密切相关,其阳性表达率高于结直肠良性肿瘤及其周围正常组织,有可能为结直肠癌的治疗提供新的靶点。
Objective To investigate the expression of VEGF-C and VEGFR-3 in colorectal cancer and to analyze the association between VEGF-C or VEGFR-3 expression and clinicopathological variables.Methods Using immunohistochemistry, VEGF-C and VEGFR-3 protein expression was detected in 63 cases of colorectal cancer tissues（experimental group） and 20 cases of benign adenoma and normal tissues（control group）.And lymphatic micro-vessel density（LMVD） was counted respectively.The relationship between VEGF-C,VEGFR-3,LMVD and the clinicopathological variables was analyzed.Results LMVD in experimental group was higher than that in the control group（14.82±4.41 vs.9.56±2.32,P 0.01）. LMVD was significantly higher in patients with lymph node metastasis,,in the later TNM stage,with positive VEGF-C and VEGFR-3 expression（P 0.01）.No significant difference was found between sex,age,histological types and differentiation （P 0.05）.The expressions of VEGF-C and VEGFR-3 in colorectal cancer were significantly higher than those in benign adenoma and normal tissues（60.32%vs.35.00%and 71.43%vs.40.00%,both P 0.01）.In the experimental group,significantly higher level of VEGF-C and VEGFR-3 was observed in the metastatic lymph nodes and in the later TNM stage tissues（P 0.01）.Nevertheless,no significant difference was found between different gender,age,histological types,and differentiation.Conclusion The expressions of VEGF-C and VEGFR-3 in colorectal cancer were higher than those in benign adenoma and normal tissues and closely correlated with tumor progression and invasiveness.This can provide new targets for colorectal cancer therapy.
出处
《疑难病杂志》
CAS
2012年第12期936-939,F0003,共5页
Chinese Journal of Difficult and Complicated Cases
基金
济南市科技发展计划资助项目(No.201003115)
