摘要
【目的】研究环氧合酶-2(COX-2)和诱导型一氧化氮合酶(iNOS)在地鼠颊囊肿瘤发生发展中的表达,探讨两者可能的相关性。【方法】90只金黄地鼠被分成两组:实验组80只,对照组10只,利用二甲基苯并蒽(9,10-dimethyl-1,2-benz-anthracene,DMBA)诱导地鼠颊囊癌变,免疫组化法检测iNOS和COX-2的动态表达。【结果】在16周时所有存活的实验组地鼠颊囊全部发生癌变,iNOS从正常黏膜、异常增生到浸润癌阳性表达率增加,分别为0%,62%,82.14%,有显著性差异;COX-2从正常黏膜、异常增生到浸润癌阳性表达率增加,分别为11.11%,16.67%,56.25%,有显著性差异;iNOS,COX-2存在呈正相关性(γ=0.279,P<0.05)。【结论】iNOS和COX-2表达在地鼠颊囊鳞癌发展中起重要作用,并呈正相关作用。
[Objective] To study the expression of inducible nitric oxide synthase （iNOS） and cyclooxygenase-2 （COX-2） and their relationship with cheek pounch carcinogenesis in golden hamster. [Method]Eighty hamsters were used as experiment group and 10 hamsters as control group. DMBA was applied on cheek pouch in the experiment group hamster but not in the control group. The expression of iNOS and COX-2 were checked in cheek pouch by immunohistochemistry with iNOS and COX-2 antibody. [Result] The expression ratio of iNOS increased gradually from normal （0%）, hyperplasia （66.67%）, to carcinoma （93.75%） in experiment group （P〈 0.05）. COX-2 expression ratio also increased gradually from normal （11.11% ）, hyperplasia （16.67%）, to carcinoma （56.25 %） in hamster pouch of experiment group. The expression of iNOS and COX-2 was correlated （γ=0.279, P〈 0.05）. [Conclusion] The expression of iNOS and COX-2 are correlated with each other and may play an important role in the development of cheek pouch carcinogenesis in golden hamster.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2007年第3期297-300,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省卫生厅基金资助项目(A2006207)
关键词
一氧化氮合酶
环氧合酶-2
颊癌
模型
nitric oxide synthase
cyclooxygenase-2
cheek carcinoma
model
