摘要
为建立高效液相色谱法测定液态奶中三聚氰胺含量方法,采取三聚氰胺用三氯乙酸溶液提取,提取液离心后经混合型阳离子交换固相萃取柱净化,洗脱物用甲醇溶液溶解,最后用高效液相色谱仪进行测定。结果表明,校准曲线在0.05 mg/kg^1.0 mg/kg范围内,相关系数r>0.999,奶与奶制品中三聚氰胺的检测限为0.5 mg/kg,定量限为1 mg/kg。高效液相色谱法测定液态奶中三聚氰胺的方法灵敏度高,适用于液态奶中三聚氰胺的测定。
To testing the malamine content in liquid milk by high permance liquid chromatography. The milk samples were extracted with trichloroacetie acid solution, extracted liquid were purified in the hybrid cation-exchange solid-phase extraction column after eentrifugation. Elution materials were soluted with methanol and were determined finally by high performance liquid chromatography. Detection limit of melamine was 0.5 mg/kg and quantitive limit was 1 mg/kg. This method was highly sensitivity,and was applicable to determine the content of melamine in the liquid milk.
出处
《动物医学进展》
CSCD
北大核心
2009年第4期57-59,共3页
Progress In Veterinary Medicine
关键词
高效液相色谱法
三聚氰胺
液态奶
high performance liquid chromatography
melamine
liquid milk
solid-phase extraction column
methanol
