摘要
2008年三聚氰胺事件后,三聚氰胺的检测对乳品企业质量控制非常重要,是乳品企业质量控制部门日常工作的重要监测项目。本文对传统的免疫学方法、液相色谱法、质谱法以及较为新颖的近红外线吸收法、拉曼光谱法、传感器、电化学等方法进行了梳理。色谱方法和免疫学方法较为常用,HPLC方法相对于LC-MS/MS或者GC-MS(GC-MS/MS),灵敏度偏低;LC-MS/MS或GC-MS(GC-MS/MS)的灵敏度较高,但仪器昂贵,检测耗时较长。免疫学检测方法是基于抗原抗体反应,受限于抗体本身的特异性,以及样品中的干扰成分,免疫学检测产品检测结果不稳定,其中胶体金试纸条使用简便,目前在行业内广泛使用,但经常会遇到假阴性、假阳性的情况;酶联免疫定量结果通常比胶体金产品更为可靠。
After the melamine incident in 2008,the detection of melamine is very important for the quality control of dairy companies.It is an important monitoring item for the daily work of the quality control department of dairy enterprises.In this paper,the traditional immunological methods,liquid chromatography,mass spectrometry,as well as the more novel near-infrared absorption method,Raman spectroscopy,sensors,electrochemistry and other methods have been combed.Chromatographic methods and immunological methods are more commonly used.Compared with LC-MS/MS or GC-MS(GC-MS/MS)methods,HPLC methods have lower sensitivity;LC-MS/MS or GC-MS(GCMS)/MS)has high sensitivity,but the instrument is expensive and the detection takes a long time.The immunological detection method is based on the antigen-antibody reaction,and is limited by the specificity of the antibody itself and the interfering components in the sample.The result of the immunological detection product is unstable.Colloidal gold test strips are easy to use and are currently widely used in the industry,but false negatives and false positives are often encountered.ELISA quantitative results are usually more reliable than colloidal gold products.Raman spectroscopy has the advantages of simple pre-processing and fast detection speed,but its sensitivity is lower than that of immunological methods.Polarography,the pre-processing is more cumbersome,and the detection sensitivity.
作者
赵瑞
杨世杰
杨艳
段玉娟
ZHAO Rui;YANG Shijie;YANG Yan;DUAN Yujuan(Kunming Xuelan Milk Co.,Ltd.,Kunming Yunnan 650217)
出处
《中国乳业》
2021年第5期69-77,共9页
China Dairy
关键词
三聚氰胺
乳制品
检测方法
melamine
dairy products
detection method
