摘要
采用RT-PCR法,从大白菜(Brassica rapasubsp.pekinensis)河头早中克隆了拟南芥CBF4的同源基因BrCBF4,并对它的序列特征、蛋白结构和遗传进化进行了分析。结果表明,BrCBF4全长663 bp,编码一个包含220个氨基酸残基和具有典型AP2结构域的蛋白,并且含有两个潜在的豆蔻酰化位点。遗传进化分析表明,BrCBF4与BjDREB1-2的关系最近,与拟南芥AtCBF4同属一个类群,而AtCBF1,2,3与大白菜的BrCBF1,2,3及其它芸薹属植物相关基因分属另一个类群。推测BrCBF4与BjDREB1-2、AtCBF4为直系同源基因,在功能上相似,而与BrCBF1,2,3存在功能上的分化。本研究为进一步研究BrCBF4在大白菜非生物逆境响应中的功能奠定了基础。
By RT- PCR, the eDNA sequence of BrCBF4 gene was isolated from Chinese cabbage （Brassica rapa subsp, pekinensis） landraee Hetouzao. BrCBF4 gene was homologous with Arabidopsis CBF4. The characteristics of BrCBF4 sequence and its predicted coding protein, also its phylogenetie relationship with other plants＇ CBFs were analyzed. The results showed that the full length coding sequence of BrCBF4 was 663 bp, and it was predicted to encode a protein with 220 aa, typical AP2 domain and two putative myrtistoylation sites. Phylogenetie analysis showed that BrCBF4 had the closest relationship with Brassica juncea gene DREB 1 -2, and belonged to the same group with AtCBF4, while Brassica rapa CBF 1 - 3 were attributed to another group with Arabidopsis CBF 1 - 3 and other related Brassica CBF/DREB genes. These results suggested that BrCBF4 was an orthologous gene of BjDREB 1 - 2 and AtCBF4, and they might have similar biological functions diverging from BrCBF 1 - 3. These results would be useful for studying functions of Chinese cabbage CBF4 gene in response to abiotic stress in the future.
出处
《山东农业科学》
2009年第4期1-4,共4页
Shandong Agricultural Sciences
基金
国家科技基础条件平台建设项目(2005DKA21001-38)
山东省农业科学院高新技术自主创新基金项目(2007YC003)
关键词
大白菜
CBF4
克隆
蛋白结构分析
遗传进化
Chinese cabbage
CBF4
Cloning
Structural analysis of protein
Phylogeny
