摘要
研究了不同磷浓度时渗透压对产甘油假丝酵母甘油合成与胞内磷积累的影响。结果表明,不同磷含量时,产甘油假丝酵母甘油合成越多,分泌至胞外和积累于胞内的甘油也越多,其最大甘油合成量存在一个最适渗透压。同样;在相同渗透压下,其最大甘油合成量也存在一个最适磷浓度。在相同磷含量时,渗透压增高能够促进胞内聚磷酸盐积累;当渗透压相同时,培养基中磷含量增加,胞内游离磷和聚磷酸盐均增加。在生长稳定期后期,富磷可以促进胞内游离磷和聚磷酸盐积累显著增加。经分析发现,产甘油假丝酵母胞内积累甘油与聚磷酸盐,可能对克服对数生长期细胞数量少而渗透压胁迫大的困境发挥了极其重要的作用,从而能维持其生长稳定期较高的生物量、细胞存活率和甘油产量。
Effects of osmotic pressure on glycerol production and intracellular phosphorus accumulation by Candida glycerinogertes at different phosphate concentrations was studied. The results showed that, at different KH2PO4 concentrations, the more yield of glycerol synthesis by C. glycerinogenes, the higher yields of extraeellular and intracellular glycerol. There is a suitable osmotolerance that the maximum glycerol production is achieved by C. glycerinogenes at the same KH2PO4 concentration. Similarly, there is a suitable phosphorus concentration to produce the maximum glycerol yields at the same osmotoleranee. The increase in osmotic pressure can promote the accumulation of intracellular polyphosphate （PolyP） at the same KH2PO4 concentration. So the increase in KH2PO4 concentration does at the same osmotic pressure. At the late of stationary phase, superfluous phosphorus can clearly enhance the accumulation of intracellular free phosphorus and PolyP. The analysis demonstrated that the accumulation of intracellular glycerol and PolyP could play an important role in overcoming the trouble of less cells and large osmotic pressure, thus maintaining its larger biomass concentration, cell survival and glycerol production.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第4期61-66,共6页
China Biotechnology
基金
湖南省自然科学基金(07JJ6031)
湖南省应用化学重点建设学科基金(2008zjxk102)资助项目
关键词
渗透压
甘油合成
胞内磷积累
产甘油假丝酵母
Osmotic pressure Glycerol production -Intracellular phosphorus accumulation Candida glycerinogenes
